Cryptic speciation and species diversity among Australian and New Zealand hornwort taxa of Megaceros (Dendrocerotaceae)
D. Christine Cargill A B D , Nicole G. F. Vella C , Ish Sharma A B and Joseph T. Miller B
+ Author Affiliations
- Author Affiliations
A Australian National Botanic Gardens, GPO Box 1777, Canberra, ACT 2601, Australia.
B Centre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia.
C Macquarie University Microscopy Unit, Faculty of Science, Macquarie University, NSW 2109, Australia.
D Corresponding author. Email: chris.cargill@environment.gov.au
Australian Systematic Botany 26(5) 356-377 https://doi.org/10.1071/SB13030
Submitted: 12 July 2013 Accepted: 25 October 2013 Published: 20 December 2013
Abstract
Recent molecular studies have redefined families and genera within the anthocerotes, but species boundaries are only now being revised. Monophyly of Australian and New Zealand taxa within the genus Megaceros Campb., which share the same spore and similar gametophyte morphology, was assessed. On the basis of sequence data, mainland Australian lineages are not monophyletic but are interspersed with New Zealand lineages. There are three species present in Australia, including the common and widespread M. gracilis (Reichardt) Steph., the widespread tropical M. flagellaris (Mitt.) Steph., recognised by its tesselated spores, and a new species, M. austronesophilus, found only in Tasmania and Macquarie Island. None of the currently recognised New Zealand taxa is conspecific with any of the Australian taxa. However, New Zealand lineages were found to be genetically and morphologically more diverse than is currently recognised.
References
Adam P (1992) ‘Australian Rainforests.’ (Oxford University Press: New York)Asthana AK, Srivastava SC (1991) ‘Indian Hornworts: a Taxonomic Study.’ (J. Cramer: Berlin)
Bayer RJ, Mabberley DJ, Morton C, Miller C, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S (2009) A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. American Journal of Botany 96, 668–685.
| A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktlGntrc%3D&md5=5fc30527d34474e5c0eb9aa90a4df686CAS | 21628223PubMed |
Campbell EO (1982) Some Anthocerotae of New Zealand with particular reference to their geographical distribution. The Journal of the Hattori Botanical Laboratory 52, 37–44.
Campbell EO (1984) Notes on some Anthocerotae of New Zealand (4). Tuatara 27, 105–120.
Campbell EO (1995) Name changes in Megaceros (Anthocerotae). New Zealand Journal of Botany 33, 279–283.
| Name changes in Megaceros (Anthocerotae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXlvV2htb8%3D&md5=6a1c6e278c419244d575d5a63ae1f26dCAS |
Cargill DC, Fuhrer BA (2008) Taxonomic studies of the Australian Anthocerotophyta II. The genus Phaeoceros. Fieldiana 47, 239–253.
| Taxonomic studies of the Australian Anthocerotophyta II. The genus Phaeoceros.Crossref | GoogleScholarGoogle Scholar |
Duff RJ, Cargill DC, Villarreal JC, Renzaglia KS (2004) Phylogenetic relationships of the hornworts based on rbcL sequence data: novel relationships and new insights. Monographs of the Missouri Botanical Gardens 98, 41–58.
Duff RJ, Villarreal JC, Cargill DC, Renzaglia KS (2007) Progress and challenges toward developing a phylogeny and classification of the hornworts. The Bryologist 110, 214–243.
| Progress and challenges toward developing a phylogeny and classification of the hornworts.Crossref | GoogleScholarGoogle Scholar |
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Frackman S, Kobs G, Simpson D, Storts D (1998) Betaine and DMSO: enhancing agents for PCR. Promega Notes Magazine 65, 27–30.
Geissler P, Bischler H (1989) Megaceros. In ‘Index Hepaticarum. Vol. 11. Naiadea to Pycoscenus’. (Eds P Geissler, H Bischler) pp. 303–306. (J. Cramer: Berlin)
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.
Hasegawa J (1983) Taxonomical studies on Asian Anthocerotae. III. Asian species of Megaceros. The Journal of the Hattori Botanical Laboratory 54, 227–240.
Hasegawa J (1993) Taxonomical studies on Asian Anthocerotae. V. A short revision of Taiwanese Anthocerotae. Acta Phytotaxonomica et Geobotanica 44, 97–112.
Hasegawa J (1995) Four tropical Asian species of Anthocerotae newly found in continental Africa. Fragmenta Floristica et Geobotanica 40, 113–122.
Hasegawa J (2002) Studies on the bryophyte flora of Vanuatu. 6. Anthocerotae. Annals of the Tsukuba Botanical Garden 21, 103–107.
Hässel de Menéndez GG (1989) Las especies de Phaeoceros (Anthocerotophyta) de América del Norte, Sud y Central; la ornamentación de sus esporas y taxonomía. Candollea 44, 717–739.
Hässel de Menéndez GG (1990) Las especies de Anthoceros y Folioceros (Anthocerotophyta) de América del Norte, Sud y Central; la ornamentación de sus esporas y taxonomía. Candollea 45, 201–220.
Hatcher RE (1965) Towards the establishment of a pure culture collection of Hepaticae. The Bryologist 58, 192–200.
McCarthy P (2003) ‘Catalogue of Australian Liverworts and Hornworts.’ Flora of Australia Supplementary Series 21. (Australian Biological Resources Study: Canberra, Australia)
McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W, Hawksworth DL, Herendeen PS, Knapp S, Marhold K, Prado J, Prud’homme van Reine WF, Smith GF, Wiersema JH, Turland NJ (Eds) (2012) ‘International Code of Nomenclature for algae, fungi, and plants (Melbourne Code), adopted by the Eighteenth International Botanical Congress Melbourne, Australia, July 2011.’ (International Association for Plant Taxonomy: Bratislava, Slovakia). Available at http://www.iapt-taxon.org/nomen/main.php [Verified September 2013]
Mitten W (1873) Marchantieae. In ‘B. Seeman’s Flora Vitiensis: A Description of the Plants of the Viti or Fiji Islands with an Account of Their History, Uses and Properties’. p. 419. (L.Reeve and Co.: London)
Pressel S, Duckett JG (2006) The parasitic ascomycete Mniaecia jungermanniae (Nees ex Fr.) Boud. induces the formation of giant perichaetia and apogamous sporophytes in leafy liverworts. Canadian Journal of Botany 84, 384–392.
| The parasitic ascomycete Mniaecia jungermanniae (Nees ex Fr.) Boud. induces the formation of giant perichaetia and apogamous sporophytes in leafy liverworts.Crossref | GoogleScholarGoogle Scholar |
Proskauer J (1957) Studies on Anthocerotales. V. Phytomorphology 7, 113–135. [Printed 1958]
Reichardt HW (1866) Diagnosen der neuen Arten von Lebermoosen, welche die Novara-Expedition mitbrachte. Verhandlungen der Zoologisch-Botanischen Gesellschaft in Wien 16, 957
Reichardt HW (1870) Reise der öterreichischen Fregatte Novara um die Erde in den Jahren 1857, 1858, 1859, unter den Befehlen des Commodore B. von Wüllerstorf-Urbair. von A. Grunow, J. Krempelhuber, H.W. Reichardt, G. Mettenius [und] J. Milde. Redigiert von Eduard Fenzl. Erster Band: Sporenpflanzen, Botanischer Theil. 1 Band, Kaiserlich-Königlichen Hof- und Staatsdruckerei, in Commission bei K. Gerold, Wien. p. 148 and plate XXIV.
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
| MrBayes 3: Bayesian phylogenetic inference under mixed models.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntlKms7k%3D&md5=d5b4bace3df34799957a4ebee94932a0CAS | 12912839PubMed |
Royal Horticultural Society (1995) ‘RHS Colour Chart.’ (The Royal Horticultural Society, London, UK)
Scott GAM (1985) ‘Southern Australian Liverworts.’ Australian Flora and Fauna Series 2. (Australian Government Publishing Service: Canberra)
Scott GAM, Bradshaw JA (1986) Australian liverworts (Hepaticae): annotated list of binomials and check-list of published species with bibliography. Brunonia 8, 1–171.
| Australian liverworts (Hepaticae): annotated list of binomials and check-list of published species with bibliography.Crossref | GoogleScholarGoogle Scholar |
Stephani F (1916) ‘Species Hepaticarum. Vol. V.’ (George and Cie: Geneva)
Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (* and other methods) Version 4. (Sinauer Associates: Sunderland, MA)
Taberlet P, Gielley L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17, 1105–1109.
| Universal primers for amplification of three non-coding regions of chloroplast DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38Xhslel&md5=6668dd736219e8a243fef04b5d4f8cf5CAS | 1932684PubMed |
Tracey JG (1982) ‘The Vegetation of the Humid Tropical Region of North Queensland.’ (CSIRO: Melbourne)
Troitsky AV, Ignatov MS, Bobrova VK, Milyutina IA (2007) Contribution of genosystematics to current concepts of phylogeny and classification of bryophytes. Biochemistry 72, 1368–1376.
| Contribution of genosystematics to current concepts of phylogeny and classification of bryophytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpvVU%3D&md5=83c6c69ba4dbb5d5e88a7dc19dc7ae49CAS | 18205621PubMed |
Vanderpoorten A, Goffinet B, Quandt D (2006) Utility of the internal transcribed spacers of the 18S–5.8S–26S nuclear ribosomal DNA in land plant systematics with special emphasis on bryophytes. In ‘Plant Genome: Biodiversity & Evolution. Volume 2B: Lower Plants’. (Eds AK Sharma, A Sharma) pp. 385–407. (Science Publishers: Enfield, NH)
Vella N (2003) The Hornwort Genus Megaceros: the Australian Connection. CPBR Summer Studentship report. (Centre for Plant Biodiversity Research, Australian National Herbarium: Canberra) Available at http://www.anbg.gov.au/cpbr/summer-scholarship/2003-projects/vella-megaceros.html [Verified 17 May 2013]
Villarreal JC, Goffinet B, Duff RJ, Cargill DC (2010a) Phylogenetic delineation of the genera Nothoceros and Megaceros. The Bryologist 113, 106–113.
| Phylogenetic delineation of the genera Nothoceros and Megaceros.Crossref | GoogleScholarGoogle Scholar |
Villarreal JC, Cargill DC, Hagborg A, Soderstrom L, Renzaglia KS (2010b) A synthesis of hornwort diversity: patterns, causes and future work. Phytotaxa 9, 150–166.
White T, Burns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In ‘PCR Protocols. A Guide to Methods and Applications’. (Eds MA Innis, DH Gelfand, JJ Sninsky, TJ White) pp. 315–322 (Academic Press: San Diego, CA)
