Mouse testicular transcriptome after modulation of non-canonical oestrogen receptor activity
M. Duliban
A D * , A. Gurgul B * , T. Szmatola B , P. Pawlicki A , A. Milon A , Z. J. Arent B , P. Grzmil C , M. Kotula-Balak B and B. Bilinska A
+ Author Affiliations
- Author Affiliations
A Department of Endocrinology, Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland.
B University Centre of Veterinary Medicine, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059, Krakow, Poland.
C Department of Genetics and Evolution Institute of Zoology, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland.
D Corresponding author. Email: michal.duliban@doctoral.uj.edu.pl
Reproduction, Fertility and Development 32(10) 903-913 https://doi.org/10.1071/RD20025
Submitted: 24 January 2020 Accepted: 8 May 2020 Published: 11 June 2020
Abstract
The aims of this study were to shed light on the role of G-protein-coupled membrane oestrogen receptor (GPER) and oestrogen-related receptor (ERR) in mouse testis function at the gene expression level, as well as the involvement of GPER and ERR in cellular and molecular processes. Male mice were injected (50 µg kg−1,s.c.) with the GPER antagonist G-15, the ERRα inverse agonist XCT 790 or the ERRβ/ERRγ agonist DY131. Next-generation sequencing (RNA-Seq) was used to evaluate gene expression. Bioinformatic analysis of read abundance revealed that 50, 86 and 171 transcripts were differentially expressed in the G-15-, XCT 790- and DY131-treated groups respectively compared with the control group. Annotated genes and their protein products were categorised regarding their associated biological processes and molecular functions. In the XCT 790-treated group, genes involved in immunological processes were upregulated. In the DY131-treated group, genes with increased expression were primarily engaged in protein modification (protein folding and small protein conjugation). In addition, the expression of genes recognised as oncogenes, such as BMI1 proto-oncogene, polycomb ring finger (Bmi1) and nucleophosphin 1 (Npm1), was significantly increased in all experimental groups. This study provides detailed information regarding the genetic changes in the testicular transcriptome of the mouse in response to modulation of non-canonical oestrogen receptor activity.
Additional keywords: G-protein-coupled oestrogen receptor, next-generation sequencing, oestrogen-related receptor, testis.
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