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Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway

Jing Wang, Yongyong Rao, Chongfan Du, Ao Wang, Yanling Wu, Ruiyi Lin, Tianfang Xiao, weimin lin

Abstract

Context. The occurrence of apoptosis due to transient oxidative damage to spermatozoa is one of the major challenges in semen preservation. At present, the addition of protective agents is the primary method of protection to alleviate damage. Aims. This study aims to elucidate the mechanism of action of cryopreservation and to discover high-quality combinatorial cryopreservation dilutions. Methods. In this study, two protective agents, bovine serum albumin (BSA) and sodium alginate (SA), were combined to determine the effectiveness of these new additives in protecting porcine sperm from damage during cryopreservation. The mechanism of action of the combination of SA and BSA was further elucidated at the molecular level. Key proteins are identified through proteomic analysis. Key results. The results showed that the sperm treated with 2 mg/ml SA and 5 mg/ml BSA had the optimal motility parameters, the best functional integrity and the most effective ability to alleviate oxidative stress. Combined with proteomic data, the results showed that it was involved in regulating the improvement of sperm quality by mediating the activity of the PI3K-AKT pathway. Conclusions. This study found that the SA and BSA combinations have an efficient protective effect on the preservation of sperm frozen. Implications. It provides theoretical technical support as a composite additive protection sperm from damage. It is of great significance to improve the quality of pig semen and improve reproduction.

RD24127  Accepted 20 January 2025

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