66 Molecular characterisation of inhibitor of apoptosis gene in native chicken of Poonch region from international borders of India and Pakistan

Apoptosis of programmed cell death is an important phenomenon of body immune response. Inhibitor of Apoptosis gene (IAP-1) is involved in different biological activities like binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. The present study was undertaken with the main objectives of molecular characterisation of IAP-1 gene in local native chicken of Poonch region. RNA Purification Kit (HiPura) was used for RNA extraction from Poonchi blood. After cDNA synthesis, IAP-1 gene of 544 bp size was amplified from cDNA using specific primers designed by Primer 3 software. Direct sequencing was carried out by Sanger sequencing with both forward and reverse primers to obtain 544 bp sequence product. The sequences were studied by using BioEdit and MEGA X softwares. The sequence results of IAP-I gene of Poonchi chicken and Kadaknath chicken were compared with, Leghorn and Fayoumi breeds of chicken and also with other sequences of Gallus gallus complete Cds, Centrocercus urophasianus, Mus musculus, Gallus gallus partial Cds, Rattus norvegicus, and Tympanuchus phasianellus. AT content were higher than GC content for IAP-1 gene. Sequence results were analysed for synonymous (S) and nonsynonymous (NS) changes. No SNP variations within Poonchi population or Kadaknath population were observed. The Fayoumi breed showed two NS changes. The highest number of NS changes were observed with Mus musculus. The genetic distance result showed no dissimilarity between Poonchi chicken and Kadaknath breed. The highest genetic distance was observed with Tympanuchus phasianellus.