102 EFFECTS OF LINOLEIC ACID/LINOLENIC ACID RATIO ON OOCYTE MATURATION AND FERTILIZATION IN PREPUBERTAL SHEEP
V. Ghaffarilaleh A , R. Ghaffarilaleh B and G. Ghaffarilaleh CA Animal Science Department, Stellenbosch University, Stellenbosch, Cape Town, South Africa;
B Veterinary Centre, Tabriz, East Azerbaijan, Iran;
C Department of Obstetrics and Gynaecology, Osku hospital, Tabriz University of Medical Sciences, Tabriz, East Azerbaijan, Iran
Reproduction, Fertility and Development 28(2) 181-181 https://doi.org/10.1071/RDv28n2Ab102
Published: 3 December 2015
Abstract
The aim of this study was to clarify the effects of linoleic acid (LA)/alpha-linolenic acid (ALA) ratio on oocyte maturation and fertilization. Prepubertal sheep ovaries were sliced, then cumulus-oocyte complexes were matured in a media based on TCM-199 supplemented with FSH, LH, epidermal growth factor, BSA fraction V, cysteamine, glutamine, and sodium pyruvate for 24 h in different ratios of LA/ALA (0, 1 : 1, 2 : 1, 4 : 1, and 10 : 1). After maturation, 169 oocytes stained for maturation rate. In the second experiment, 176 presumptive zygotes from 227 fertilized oocytes were stained for fertilization rate. In the third experiment, 570 oocytes were used for embryo development. The generalized linear model was used for analysis. The results showed that LA/ALA at the ratios of 1 : 1, 2 : 1, and 4 : 1 significantly (P < 0.05) improved the MII percentages and the 10 : 1 ratio was detrimental. Fertilization rate was not significant between treatments, but the two pro-nuclei percentage was significantly increased for the ratios of 1 : 1, 2 : 1, and 4 : 1 (P < 0.05). Cleavage rate for the ratio of 2 : 1 and 4 : 1 was significantly higher (P < 0.05). The ratio of 10 : 1 was detrimental for embryo development, and none of the cleaved embryos reached the blastocyst rate. In conclusion, the ratio of 10 : 1 LA/ALA was detrimental and resulted in degenerated oocytes and subsequently for prepubertal sheep embryos, whereas lower ratios of LA/ALA were beneficial for oocyte competition and fertilisation.
