153 IN VITRO CULTIVATION OF CAPRINE EMBRYOS PRODUCED IN VIVO
A. C. Martinez A , R. C. M. Tramontini A , M. A. Saito Jr A , C. O. Abreu A and C. R. Alcalde BA Universidade Estadual de Maringá, Campus Avançado de Umuarama, Umuarama, Paraná, Brazil;
B Universidade Estadual de Maringá, Campus Sede, Maringá, Paraná, Brazil
Reproduction, Fertility and Development 22(1) 235-235 https://doi.org/10.1071/RDv22n1Ab153
Published: 8 December 2009
Abstract
Independent of embryo stage, when grade III embryos are transferred, they usually provide poor pregnancy rates. The aim of this study was to compare the effect of different in vitro cultivation media on the development of in vivo derived grade III caprine embryos. Twelve adult crossbreed goats from the Animal Breeding and Reproduction Laboratory of Maringá State University were used for this experiment. The goats were not pregnant or lactating. After embryo collection, embryos were classified regarding their development and quality, according to the International Embryo Transfer Society manual. Morulae classified as grade III were used in this experiment. The embryos were cultivated in 2 different media: Holding Plus medium (Bioniche Animal Health, Belleville, Ontario, Canada) or PBS plus 10% of bovine fetal serum (Nutricell, Campinas, Brazil) in 38.5°C waterbath with water circulation for 24 hours. After 24 h, the embryos were morphologically assessed. The percentage of embryos that developed during the cultivation period was calculated to evaluate the effectiveness of culture media. The embryos that produced new cell divisions and changed from grade III (poor quality) to grade II or grade I (excellent or good) were considered developed embryos. Chi-square was used to determine statistical differences between media. In the present work, the rate of embryos that developed in Holding Plus medium (Bioniche Animal Health) was 75%, and with PBS plus 10% bovine fetal serum was 40% of the total cultivated embryos.
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