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Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Culture of preantral ovarian follicles in the grey, short-tailed opossum, Monodelphis domestica

L Butcher and SL Ullmann

Reproduction, Fertility and Development 8(4) 535 - 539
Published: 1996

Abstract

A culture system designed to support the development of individual preantral mouse ovarian follicles has been employed to study follicle growth in the New World marsupial species Monodelphis domestica. Preantral follicles were isolated mechanically and cultured individually in microdrops under oil. Preliminary results indicate that follicle growth was positively correlated to the concentration of follicle-stimulating hormone (FSH) provided, with 1.0-1.5 IU FSH mL-1 producing the best results. Incubation at the body temperature of M. domestica (33 degrees C) was found to be preferable to that at 37 degrees C. The culture system was able to support follicle growth; however, despite follicles exceeding the size when antrum formation occurs in vivo, they remained preantral.

https://doi.org/10.1071/RD9960535

© CSIRO 1996

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