Differential expression and localisation of heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) in the testis and epididymis of Sonid Bactrian camels
Gaowa Hasi A , Liyasu Wu B , Tserennadmid Sodnompil A , Ruhan Yi A , Rihan Wu A , Rui Zhang A , Haya Na A , Hejie Liu A , Musi Ji A , Wangwei Xie A and Narenhua Nasenochir
A C *
+ Author Affiliations
- Author Affiliations
A College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
B College of Animal Breeding and Genetics, Norwegian University of Life Sciences, Ås, Akershus, Norway.
C Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
Reproduction, Fertility and Development 35(10) 552-562 https://doi.org/10.1071/RD23026
Published online: 9 June 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Context: Heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) are biomarkers of oxidative stress and stress in temperate, tropical environments, which are crucial for male reproduction. Their expression and distribution patterns in the testis and epididymis of Bactrian camels are still unknown.
Aims: This study aims to investigate the HSP70 and GPX5 expression and localisation in 3- and 6-year-old Bactrian camel testis and epididymis.
Methods: Reverse transcription quantitative polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry were used to detect HSP70 in the testis and epididymis (caput, corpus and cauda) and GPX5 in the epididymis at two developmental stages (3-year-old puberty group and 6-year-old adult group).
Key results: HSP70 was upregulated in the testis. Immunohistochemistry results indicated the HSP70 protein was mainly detected in spermatids and Leydig cells of testicular tissue. In the epididymis, HSP70 was located at the luminal spermatozoa, the epithelium lining the epididymal and the epididymal interstitium. GPX5 expression was significantly higher in the caput epididymis than in the corpus and cauda epididymis. GPX5 protein was observed in the epithelium lining the epididymal, interstitium and luminal spermatozoa in the epididymis by immunohistochemistry.
Conclusions: Bactrian camel HSP70 and GPX5 exhibited spatiotemporal expression specificity.
Implications: HSP70 and GPX5 may be essential for germ cell development and reproductive success after sexual maturation in Sonid Bactrian camels.
Keywords: Bactrian camels, epididymis, glutathione peroxidase 5, heat shock protein 70, immunohistochemistry, qRT-PCR, testis, Western blot.
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