Effects of transforming growth factor β1 on steroidogenesis of feline granulosa cells cultured in vitro
Excel R. S. Maylem A and Leon J. Spicer
A *
+ Author Affiliations
- Author Affiliations
A Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Reproduction, Fertility and Development 34(11) 789-797 https://doi.org/10.1071/RD22034
Published online: 24 May 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: Little is known about the hormonal regulation of feline ovarian granulosa cell proliferation and steroidogenesis.
Aims: To determine if transforming growth factor β1 (TGFB1), activin, epidermal growth factor (EGF), follicle stimulating hormone (FSH), luteinizing hormone (LH), melatonin, and insulin-like growth factor 1 (IGF1) regulate granulosa cell steroidogenesis and proliferation in cats, three experiments were conducted in winter season.
Methods: Granulosa cells were isolated and treated in vitro with various hormones in serum-free medium for 48 h after an initial 48 h plating in 10% fetal calf serum.
Key results: Treatment with IGF1 and FSH increased (P < 0.05) estradiol production by 2.3- and 1.33-fold, respectively. In contrast, TGFB1 blocked (P < 0.05) IGF1-induced estradiol production and inhibited FSH-induced estradiol production by 60%. Combined with FSH or FSH plus IGF1, TGFB1 inhibited (P < 0.05) cell proliferation, whereas TGFB1 increased progesterone production by 2.8-fold in the presence of FSH plus IGF1. EGF decreased (P < 0.05) FSH plus IGF1-induced estradiol production by 89% but did not affect progesterone production or cell numbers. Activin did not affect (P > 0.10) cell numbers or steroidogenesis in the presence of FSH plus IGF1. Melatonin and LH decreased (P < 0.05) estradiol production 53% and 59%, respectively, without affecting progesterone production or cell proliferation.
Conclusions: The present study has identified TGFB1 as a major regulator of feline ovarian function, in addition to EGF, IGF1, melatonin, LH and FSH.
Implications: These studies will provide useful information for future development of fertility control in feline species.
Keywords: activin, epidermal growth factor, estradiol, feline, granulosa cell, melatonin, steroidogenesis, transforming growth factor B1.
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