In vitro effects of Type I interferons (IFNτ and IFNα) on bovine hepatocytes cultured with or without Kupffer cells
Kai Josef Endriß A , Marie Margarete Meyerholz B , Teresa Fischbach A , Lutz Brimmers A , Christiane Pfarrer C , Christina Deborah Marth D and Marion Schmicke
A E F
+ Author Affiliations
- Author Affiliations
A University of Veterinary Medicine Hanover, Clinic for Cattle, Endocrinology, Bischofsholer Damm 15, 30539 Hanover, Germany.
B Clinic for Ruminants with Ambulatory and Herd Health Services, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstraße 16, 85764 Oberschleißheim, Germany.
C University of Veterinary Medicine Hanover, Anatomy, Bischofsholer Damm 15, 30539 Hanover, Germany.
D Melbourne Veterinary School, The University of Melbourne, 250 Princes Highway, Werribee, Vic. 3030, Australia.
E Martin-Luther University Halle-Wittenberg, Faculty of Natural Sciences III, Institute of Agricultural and Nutritional Sciences, Animal Health Management, Theodor-Lieser-Straße 11, 06120 Halle, Germany.
F Corresponding author. Email: marion.schmicke@landw.uni-halle.de
Reproduction, Fertility and Development 33(4) 305-317 https://doi.org/10.1071/RD20278
Submitted: 16 October 2020 Accepted: 17 December 2020 Published: 12 February 2021
Journal Compilation © CSIRO 2021 Open Access CC BY-NC-ND
Abstract
In cattle, maternal recognition of early pregnancy depends on the effects of the embryonic signal interferon (IFN)-τ. IFN-stimulated genes have been upregulated in the maternal liver during early pregnancy. In this study, primary hepatocyte cell culture models were evaluated for their suitability to test Type I IFN effects in vitro. The expression of target genes (interferon-stimulated gene 15 (ISG-15), interferon-induced GTP-binding protein (MX-1), C-X-C motif chemokine 10 (CXCL-10), CXCL-5, insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP-2)) was measured using reverse transcription–quantitative polymerase chain reaction in hepatocytes from monoculture or in indirect coculture with Kupffer cells (HKCid) on Days 1, 2, 3 and 4 of culture (n = 21 donor cows). Gene expression was also measured on Day 4 after challenging the cultures with recombinant IFNτ, IFNα, progesterone (P4), IFNτ + IFNα or IFNτ + P4 for 6 h. A significant increase in the mRNA expression of target genes in hepatocytes was shown in response to stimulation with IFNτ. The Kupffer cells in coculture did not influence the effects of IFNτ in hepatocytes. In conclusion, primary bovine hepatocyte cultures are suitable for stimulation experiments with Type I IFNs and as an extrauterine model for embryo–maternal communication. The proposed endocrine action of IFNτ in the liver may affect maternal metabolism and immune function in the liver.
Keywords: cattle, cell culture, embryo, embryo-maternal communication, endocrinology, gestation, hepatocytes, IGF binding protein, insulin-like growth factor, interferone tau
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