Preliminary evaluation of urinary cytology and running wheel activity to detect oestrus and the effect of daily handling on breeding success of fat-tailed dunnarts Sminthopsis crassicaudata
Paige Swinston A , Stephen D. Johnston
A , Patricia O’Hara A , Robert Englebright A and Tamara Keeley A B
+ Author Affiliations
- Author Affiliations
A Wildlife Science Unit, School of Agriculture and Food Sciences, The University of Queensland, Gatton, Qld 4343, Australia.
B Corresponding author. Email: t.keeley@uq.edu.au
Reproduction, Fertility and Development 32(13) 1108-1115 https://doi.org/10.1071/RD20001
Submitted: 2 January 2020 Accepted: 15 July 2020 Published: 28 August 2020
Abstract
Eight recently weaned female fat-tailed dunnarts (Sminthopsis crassicaudata) were divided into two experimental groups: those in Group A (n = 4) received limited handling, whereas those in Group B (n = 4) were handled daily for the collection of urine samples to detect changes in cornified epithelial cells (CEC). Daily faecal output, food consumption, running wheel activity and weekly bodyweight changes were recorded. Faecal corticosterone metabolite (FCM) concentrations were determined over two periods, namely after introduction to either a new enclosure or a male for mating. Although changes in faecal output, food consumption, bodyweight and running wheel activity showed no relationship to oestrus, CEC scoring was a suitable means for detecting oestrus. Overall mean FCM concentrations did not differ significantly (P > 0.05) between the Groups A and B, and pouch young were produced in both (Group A, n = 2; Group B, n = 3), suggesting that daily handling is not a significant factor affecting breeding success. However, females that did not produce young during the study period demonstrated higher baseline FCM concentrations than females that did (mean (±s.e.m.) 79.8 ± 4.0 vs 62.3 ± 3.1 ng g−1). Daily handling for urine collection for CEC scoring appears to have little effect on adrenal output or reproductive success, confirming its utility as a reproductive management tool for this species.
Additional keywords: faecal corticosterone, oestrous cycle, urogenital cytology, video surveillance.
References
Bennett, J. H., Breed, W. G., Hayman, D. L., and Hope, R. M. (1990). Reproductive and genetical studies with a laboratory colony of the dasyurid marsupial Sminthopsis crassicaudata. Aust. J. Zool. 37, 207–222.| Reproductive and genetical studies with a laboratory colony of the dasyurid marsupial Sminthopsis crassicaudata.Crossref | GoogleScholarGoogle Scholar |
Breed, W. G., Leigh, C. M., and Bennett, J. H. (1989). Sperm morphology and storage in the female reproductive tract of the fat-tailed dunnart, Sminthopsis crassicaudata (Marsupialia: Dasyuridae). Gamete Res. 23, 61–75.
| Sperm morphology and storage in the female reproductive tract of the fat-tailed dunnart, Sminthopsis crassicaudata (Marsupialia: Dasyuridae).Crossref | GoogleScholarGoogle Scholar | 2744705PubMed |
Czarny, N. A., Garnham, J. I., Harris, M. S., and Rodger, J. C. (2009). Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata). Reproduction 138, 23–31.
| Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata).Crossref | GoogleScholarGoogle Scholar | 19380426PubMed |
Fadem, B. H. (1985). Evidence for the activation of female reproduction by males in a marsupial, the gray short-tailed ppossum (Monodeiphis domestica). Biol. Reprod. 33, 112–116.
| Evidence for the activation of female reproduction by males in a marsupial, the gray short-tailed ppossum (Monodeiphis domestica).Crossref | GoogleScholarGoogle Scholar | 4063435PubMed |
Fadem, B. H., and Rayve, R. S. (1985). Characteristics of the oestrous cycle and influence of social factors in grey short-tailed opossums (Monodelphis domestica). J. Reprod. Fertil. 73, 337–342.
| Characteristics of the oestrous cycle and influence of social factors in grey short-tailed opossums (Monodelphis domestica).Crossref | GoogleScholarGoogle Scholar | 4039362PubMed |
Godfrey, G. K. (1969). Reproduction in a laboratory colony of the marsupial mouse Sminthopsis larapinta (Marsupialia: Dasyuridae). Aust. J. Zool. 17, 637–654.
Godfrey, G. K. (1975). A study of oestrus and fecundity in a laboratory colony of mouse opossums (Marmosa robinsoni). J. Zool. 175, 541–555.
| A study of oestrus and fecundity in a laboratory colony of mouse opossums (Marmosa robinsoni).Crossref | GoogleScholarGoogle Scholar |
Godfrey, G. K., and Crowcroft, P. (1971). Breeding the fat-tailed marsupial mouse Sminthopsis crassicaudata in captivity. Int. Zoo Yearb. 11, 33–38.
| Breeding the fat-tailed marsupial mouse Sminthopsis crassicaudata in captivity.Crossref | GoogleScholarGoogle Scholar |
Hume, I. D. (1982). ‘Digestive Physiology and Nutrition of Marsupials.’ (Cambridge University Press: Cambridge.)
Hume, I. D., Smith, C., and Woolley, P. A. (2000). Anatomy and physiology of the gastrointestinal tract of the Julia Creek dunnart, Sminthopsis douglasi (Marsupialia: Dasyuridae). Aust. J. Zool. 48, 475–485.
| Anatomy and physiology of the gastrointestinal tract of the Julia Creek dunnart, Sminthopsis douglasi (Marsupialia: Dasyuridae).Crossref | GoogleScholarGoogle Scholar |
Jackson, S. (2003). ‘Australian Mammals: Biology and Captive Management.’ (CSIRO Publishing: Melbourne.)
Keeley, T., McGreevy, P. M., Fanson, B. G., Masters, K., and O’Brien, J. K. (2012). The reproductive cycle of the Tasmanian devil (Sarcophilus harrisii) and factors associated with reproductive success in captivity. Gen. Comp. Endocrinol. 176, 182–191.
| The reproductive cycle of the Tasmanian devil (Sarcophilus harrisii) and factors associated with reproductive success in captivity.Crossref | GoogleScholarGoogle Scholar | 22306283PubMed |
Lambert, C., and Mills, H. (2006). Husbandry and breeding of the dibbler Parantechinus apicalis at Perth Zoo. Int. Zoo Yearb. 40, 290–301.
| Husbandry and breeding of the dibbler Parantechinus apicalis at Perth Zoo.Crossref | GoogleScholarGoogle Scholar |
Martin, P. G. (1965). The potentialities of the fat-tailed marsupial mouse, Sminthopsis crassicaudata (Gould), as a laboratory animal. Aust. J. Zool. 13, 559–562.
| The potentialities of the fat-tailed marsupial mouse, Sminthopsis crassicaudata (Gould), as a laboratory animal.Crossref | GoogleScholarGoogle Scholar |
Morton, S. R. (1978). An ecological study of Sminthopsis crassicaudata (Marsupialia: Dasyuridae) III. Reproduction and life history. Aust. Wildl. Res. 5, 183–211.
| An ecological study of Sminthopsis crassicaudata (Marsupialia: Dasyuridae) III. Reproduction and life history.Crossref | GoogleScholarGoogle Scholar |
Palme, R. (2005). Measuring fecal steroids: guidelines for practical application. Ann. N. Y. Acad. Sci. 1046, 75–80.
| Measuring fecal steroids: guidelines for practical application.Crossref | GoogleScholarGoogle Scholar | 16055844PubMed |
Perret, M., and Ben M’Barek, S. (1991). Male influence on oestrous cycles in female woolly opossum (Caluromys philander). J. Reprod. Fertil. 91, 557–566.
| Male influence on oestrous cycles in female woolly opossum (Caluromys philander).Crossref | GoogleScholarGoogle Scholar | 2013879PubMed |
Poiley, S. M. (1975). Evaluation of requirements for defined laboratory animals in biomedical research. Adv. Pharmacol. 12, 125–184.
Pollock, K., Booth, R., Wilson, R., Keeley, T., Grogan, K., Kennerley, P., and Johnston, S. D. (2010). Oestrus in the Julia Creek dunnart (Sminthopsis douglasi) is associated with wheel running behaviour but not necessarily changes in body weight, food consumption or pouch morphology. Anim. Reprod. Sci. 117, 135–146.
| Oestrus in the Julia Creek dunnart (Sminthopsis douglasi) is associated with wheel running behaviour but not necessarily changes in body weight, food consumption or pouch morphology.Crossref | GoogleScholarGoogle Scholar | 19362793PubMed |
Roberts, C. T., and Breed, W. G. (1994). Placentation in the dasyurid marsupial, Sminthopsis crassicaudata, the fat-tailed dunnart, and notes on placentation of the didelphid, Monodelphis domestica. J. Reprod. Fertil. 100, 105–113.
| Placentation in the dasyurid marsupial, Sminthopsis crassicaudata, the fat-tailed dunnart, and notes on placentation of the didelphid, Monodelphis domestica.Crossref | GoogleScholarGoogle Scholar | 8182576PubMed |
Selwood, L. (1987). Embryonic development in culture of two dasyurid marsupials, Sminthopsis crassicaudata (Gould) and Sminthopsis macroura (Spencer), during cleavage and blastocyst formation. Gamete Res. 16, 355–371.
| Embryonic development in culture of two dasyurid marsupials, Sminthopsis crassicaudata (Gould) and Sminthopsis macroura (Spencer), during cleavage and blastocyst formation.Crossref | GoogleScholarGoogle Scholar | 3506922PubMed |
Smith, M. J., Bennett, J. H., and Chesson, C. M. (1978). Photoperiod and some other factors affecting reproduction in female Sminthopsis crassicaudata (Gould) (Marsupialia: Dasyuridae) in captivity. Aust. J. Zool. 26, 449–463.
| Photoperiod and some other factors affecting reproduction in female Sminthopsis crassicaudata (Gould) (Marsupialia: Dasyuridae) in captivity.Crossref | GoogleScholarGoogle Scholar |
Swinston, P., Johnston, S. D., Phillips, C. J. C., O’Hara, P., Murray, P., and Keeley, T. (2015). Evaluation and optimization of non-invasive hormone analysis techniques in the fat-tailed dunnart. In ‘Australian Mammal Society Abstracts. 2015 Scientific Meeting’, 6–10 July 2015, Hobart, Tas., Australia. pp. 38. [Abstract] (Australian Mammal Society: Hobart.)
Warnecke, L., Körtner, G., Burwell, C. J., Turner, J. M., and Geiser, F. (2012). Short-term movement patterns and diet of small dasyurid marsupials in semiarid Australia. Aust. Mammal. 34, 49–54.
| Short-term movement patterns and diet of small dasyurid marsupials in semiarid Australia.Crossref | GoogleScholarGoogle Scholar |
Woolley, P. A. (1990). Reproduction in Sminthopsis macroura (Marsupialia: Dasyuridae) I. The female. Aust. J. Zool. 38, 187–205.
| Reproduction in Sminthopsis macroura (Marsupialia: Dasyuridae) I. The female.Crossref | GoogleScholarGoogle Scholar |
Woolley, P. A. (2007). Observations on the oestrous cycle and breeding season of the chestnut dunnart Sminthopsis archeri, a little known dasyurid marsupial. Aust. Mammal. 29, 89–96.
| Observations on the oestrous cycle and breeding season of the chestnut dunnart Sminthopsis archeri, a little known dasyurid marsupial.Crossref | GoogleScholarGoogle Scholar |
Woolley, P. A., and Gilfillan, S. L. (1991). Confirmation of polyoestry in captive white-footed dunnarts, Sminthopsis leucopus (Marsupialia: Dasyuridae). Aust. Mammal. 14, 137–138.
Woolley, P. A., and Valente, A. (1986). Reproduction in Sminthopsis longicaudata (Marsupialia: Dasyuridae): laboratory observations. Aust. Wildl. Res. 13, 7–12.
| Reproduction in Sminthopsis longicaudata (Marsupialia: Dasyuridae): laboratory observations.Crossref | GoogleScholarGoogle Scholar |
Woolley, P. A., and Watson, M. R. (1984). Observations on a captive outdoor breeding colony of a small dasyurid marsupial, Sminthopsis crassicaudata. Aust. Wildl. Res. 11, 249–254.
| Observations on a captive outdoor breeding colony of a small dasyurid marsupial, Sminthopsis crassicaudata.Crossref | GoogleScholarGoogle Scholar |
