Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
María Belén Rodríguez
A B , Andrés Gambini A B , Gabriel Clérico B C , Amada Eugenia Ynsaurralde-Rivolta D , Olinda Briski A B , Hernan Largel E , Marina Sansinena B C and Daniel F. Salamone A B F
+ Author Affiliations
- Author Affiliations
A Laboratorio de Biotecnología Animal, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martin 4453, Agronomía, C1417DSE CABA, Buenos Aires, Argentina.
B Consejo Nacional de Investigaciones Científicas y Técnicas, (CONICET) Godoy Cruz 2290, C1425FQB CABA, Argentina.
C Laboratorio de Biotecnología y Reproducción Animal, Facultad de Ingeniería y Ciencias Agrarias, Pontificia Universidad Católica Argentina, Av. Alicia Moreau de Justo 1300, CABA, Buenos Aires, Argentina.
D Instituto Nacional de Tecnología Agropecuaria (INTA), Juan Pujol al Este s/n, 3470 Mercedes, Corrientes, Argentina.
E Private practice at El palenque Embriones equine embryo transfer center, Est, Acceso a Lucas Monteverde, B6660 Veinticinco de Mayo, Provincia de Buenos Aires, Argentina.
F Corresponding author. Email: salamone@agro.uba.ar
Reproduction, Fertility and Development 31(12) 1805-1811 https://doi.org/10.1071/RD19248
Submitted: 2 July 2019 Accepted: 27 October 2019 Published: 21 November 2019
Abstract
Assisted reproduction techniques (ARTs) have become widespread in the equine breeding industry. In particular, the combination of oocyte recovery from live mares followed by IVM and intracytoplasmic sperm injection (ICSI) has increased markedly among the ARTs used with valuable or low-fertility animals. There is currently no consensus among research groups regarding the optimal oocyte maturation period to produce high-quality embryos. In this study, we report the maturation dynamics of equine oocytes at different time points, from 20 to 40 h (Experiment 1). In addition, in Experiment 2, equine ICSI blastocysts were produced from oocytes that exhibited early (up to 24 h) or late (28–30 h) extrusion of the first polar body (PB). Blastocyst rates and diameter were recorded and embryo quality was assessed by analysing the number of apoptotic cells and Yes-associated protein 1 (YAP1) expression. By 20 h of IVM, 42% of oocytes were mature, and the remaining oocytes matured within the next 17 h of IVM. Although no differences were found in cell apoptosis or the number of YAP1-positive cells between groups exhibiting early and late PB extrusion, embryos from the early group (Group I) exhibited an improved total cell number and blastocyst rate compared to embryos from the late group (Group II) (18.60% vs 10.17% respectively).
Additional keywords: blastocyst, embryo, in vitro maturation, IVM, mare, nucleus.
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