Leukaemia inhibitory factor increases αvβ3 integrin expression in cultured mouse blastocysts
Ali Hosseini A , Nassim Ghorbanmehr B , Mojtaba Rezazadeh Valojerdi C , Mehrdad Bakhtiyari A E and Bahar Movaghar
D E
+ Author Affiliations
- Author Affiliations
A Department of Anatomy, Faculty of Medicine, Iran University of Medical Science, Shahid Hemmat Highway, Tehran, P.O. Box 1449614535, Iran.
B Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, North Sheikh Bahaee Street, Deh-e Vanak, Tehran, P.O. Box 1993891176, Iran.
C Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, Jalal AleAhmad, Nasr, Tehran, P.O. Box 14115-111, Iran.
D Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, No. 2, Hafez Street, Banihashem Street, Resalat Avenue, Tehran, P.O. Box 16635-148, Iran.
E Corresponding authors. Emails: b.movaghar@royaninstitute.org; mehr_bakhtiyari@yahoo.com
Reproduction, Fertility and Development 32(13) 1116-1124 https://doi.org/10.1071/RD19183
Submitted: 21 May 2019 Accepted: 16 February 2020 Published: 12 August 2020
Abstract
Blocking leukaemia inhibitory factor (LIF) has a negative effect on the implantation of mouse embryos. It has been determined that LIF regulates the expression of endometrial αvβ3 integrin, but its role in trophoblast cells remains unclear. The aim of this study was to evaluate the effect of 1000 units mL−1 LIF on the expression of LIF receptor alpha (Lifr), integrin alpha V (Itgav), integrin beta 3 (Itgb3) and some apoptosis-related genes in blastocysts formed from 8-cell mouse embryos. Embryos obtained from superovulated NMRI mice were divided into four groups and cultured to the blastocyst stage. Groups 1 and 2 were simple embryo cultures in the absence and presence of LIF respectively; Groups 3 and 4 were embryo cocultures in the absence and presence of LIF respectively. The number of cells in blastocysts was counted and the expression of Itgav, Itgb3 and some apoptosis-related genes was determined using real-time polymerase chain reaction. The blastocyst rate, cell count, survival rate and expression of the Itgav and Itgb3 genes were significantly higher in the coculture groups and in the simple culture group with LIF than in the simple culture group without LIF. In conclusion, LIF improves the growth and cell count of blastocysts, increases the expression of the Itgav and Itgb3 genes in blastocysts and plays an important role in the success of implantation.
Additional keywords: culture medium, development, gene expression, growth factor, implantation.
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