DAAM1 and PREP are involved in human spermatogenesis
Massimo Venditti
A * , Chiara Fasano B * , Sergio Minucci A D , Ismene Serino A , Antonio Agostino Sinisi C , Brian Dale B and Loredana Di Matteo A
+ Author Affiliations
- Author Affiliations
A Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate ‘F, Bottazzi’ Università degli Studi della Campania ‘Luigi Vanvitelli,’ via Costantinopoli, 16, 80138 Napoli, Italy.
B Centro Fecondazione Assistita (CFA-Italia), Via Tasso, 480, 80123, Napoli, Italy.
C Dipartimento di Scienze Mediche e Chirurgiche Avanzate, Università degli Studi della Campania ‘Luigi Vanvitelli,’ P.zza L. Miraglia, 2, 80138 Napoli, Italy.
D Corresponding author. Email: sergio.minucci@unicampania.it
Reproduction, Fertility and Development 32(5) 484-494 https://doi.org/10.1071/RD19172
Submitted: 9 May 2019 Accepted: 7 August 2019 Published: 24 January 2020
Abstract
During differentiation of the male gamete, there is a massive remodelling in the shape and architecture of all the cells in the seminiferous epithelium. The cytoskeleton, as well as many associated proteins, plays a pivotal role in this process. To better characterise the factors involved, we analysed two proteins: the formin, dishevelled-associated activator of morphogenesis 1 (DAAM1), which participates in the regulation of actin polymerisation, and the protease, prolyl endopeptidase (PREP), engaged in microtubule-associated processes. In our previous studies we demonstrated their involvement in cytoskeletal dynamics necessary for correct postnatal development of the rat testis. Here, we used samples of testicular tissue obtained from infertile men by testicular sperm extraction and the spermatozoa of asthenoteratozoospermic patients. By western blot and immunofluorescent analysis, we found that DAAM1 and PREP expression and localisation were impaired in both the testis and spermatozoa, and in particular in the midpiece as well as in the principal and end-pieces of the flagella, as compared with spermatozoa of normospermic men. Our results provide new knowledge of the dynamics of spermatogenesis, raising the possibility of using DAAM1 and PREP as new markers of normal fertility.
Additional keywords: fertility, spermatozoa, testis.
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