Betaine-loaded CaCO3 microparticles improve survival of vitrified feline preantral follicles through higher mitochondrial activity and decreased reactive oxygen species
D. C. C. Brito
A B C H , S. F. S. Domingues B , A. P. R. Rodrigues C , L. M. Silva C , K. A. Alves C , X. Wu D E , T. S. Francisco F G , I. L. Barroso Neto F , V. N. Freire F , J. R. Figueiredo C , J. C. Pieczarka A and R. R. Santos B
+ Author Affiliations
- Author Affiliations
A Laboratory of Cytogenetics, Center for Advanced Studies in Biodiversity, Biological Sciences Institute, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
B Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
C Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil.
D Hubert Department of Global Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd, Atlanta, GA 30322, USA.
E Department of Infectious Disease, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Yuhangtang Road 866, Hangzhou, Zhejiang, PR China.
F Department of Physics, Federal University of Ceará, Av. da Universidade, 2853 - Benfica, 60020-181, Fortaleza - CE, Brazil.
G Department of Chemistry, State University of Vale do Acarau, Av. da Universidade, 850 - Jerônimo de Medeiros Prado, Sobral - CE, 62010-295, Brazil.
H Corresponding author. Email: daniellecalado@ymail.com
Reproduction, Fertility and Development 32(5) 531-537 https://doi.org/10.1071/RD19068
Submitted: 27 February 2019 Accepted: 21 August 2019 Published: 24 February 2020
Abstract
Ovary fragments from six sexually mature cats were vitrified in the presence or absence of betaine or ascorbic acid, loaded (7.4 or 74 µM betaine; 20 or 200 µM ascorbic acid) or not (1 mM betaine or 0.3 mM ascorbic acid) into CaCO3 microparticles, and assessed for follicular morphology, oxidative stress and mitochondrial activity Feline ovarian tissue was successfully preserved after vitrification in the presence of 74 µM betaine loaded in CaCO3 microparticles, as confirmed by morphological analysis and the density of preantral follicles and stromal cells, as well as by the increased mitochondrial activity and decreased production of reactive oxygen species.
Additional keywords: ascorbic acid, cat, microencapsulation, osmoregulator, ovary.
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