Resveratrol directly affects ovarian cell sirtuin, proliferation, apoptosis, hormone release and response to follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I)
Alexander Sirotkin A B E , Richard Alexa A , Attila Kádasi C , Erika Adamcová A , Saleh Alwasel D and Abdel Halim Harrath D
+ Author Affiliations
- Author Affiliations
A Constantine the Philosopher University, 949 74 Nitra, Slovakia.
B Department of Genetics and Reproduction, Research Institute of Animal Production, 951 41 Lužianky, Slovakia.
C Department of Animal Physiology, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia.
D King Saud University, Department of Zoology, College of Science, Riyadh 11451, Saudi Arabia.
E Corresponding author. Email: asirotkin@ukf.sk
Reproduction, Fertility and Development 31(8) 1378-1385 https://doi.org/10.1071/RD18425
Submitted: 26 October 2018 Accepted: 12 February 2019 Published: 12 April 2019
Abstract
The objective of our study was to examine the influence of the plant polyphenol resveratrol (R) on the rapamycin signalling pathway (mammalian target of rapamycin; mTOR) and basic ovarian cell functions in mammalian targets, as well as on their response to the physiological hormonal stimulators follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I). Resveratrol was found to stimulate sirtuin 1 accumulation and apoptosis, inhibit proliferation, suppress P and promote T and E release. Alone, FSH promoted proliferation and had no effect on apoptosis, but had an inhibitory effect on these processes when combined with R. IGF-I alone stimulated proliferation and inhibited apoptosis and promoted P production but not that of T; however, in the presence of R, the addition of IGF-I switched from having an anti-apoptotic to a pro-apoptotic effect and stimulated T release, but it did not modify the effect of IGF-I on proliferation and P output. These observations: (1) demonstrate that R directly affects the basic ovarian cell functions of proliferation, apoptosis and steroidogenesis, (2) provide further evidence of the involvement of FSH and IGF-I in the regulation of these processes, (3) demonstrate the ability of R to prevent and even invert the effects of FSH and IGF-I on ovarian cells and (4) indicate that the effects of R may be mediated by the mTOR–sirtuin intracellular signalling system.
Additional keywords: bax, caspase 3, granulosa cells, mTOR–sirtuin, ovarian follicle, PCNA, progesterone, testosterone.
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