Amelioration of sperm immobilisation factor-induced infertility by bacterial antigenic determinants cross-reacting with spermatozoa
Deepali Thaper A , Deepak K. Rahi A and Vijay Prabha
A B
A Department of Microbiology, Panjab University, Chandigarh-160014, India.
B Corresponding author. Email: satishvijay11@yahoo.com
Reproduction, Fertility and Development 31(3) 602-612 https://doi.org/10.1071/RD18300
Submitted: 9 February 2018 Accepted: 16 September 2018 Published: 6 December 2018
Abstract
A strain of Staphylococcus aureus, capable of in vitro immobilisation of human and mouse spermatozoa, was already present in our laboratory. Therefore, in the present study, the factor responsible (sperm immobilisation factor, SIF) was isolated and purified. It was found to compromise not only motility, but also viability, morphology and Mg2+-ATPase activity of mouse spermatozoa. Also, SIF (250 μg mL−1), when administered intravaginally in female BALB/c mice before mating, showed 100% contraceptive effect. Moreover, fluorescein isothiocyanate-labelled SIF was also found to bind mouse spermatozoa and various motile as well as non-motile bacteria, indicating the presence of common SIF-binding receptors on spermatozoa and bacteria. Further, to demonstrate molecular mimicry, the amelioration of SIF-induced impairment of sperm function by a SIF-binding bacterial receptor was compelling. For this, the SIF-binding receptor from Escherichia coli (E-SBR) was purified and evaluated for its ameliorative effect on SIF-induced sperm impairment in vitro and in vivo. Interestingly, upon the addition of mouse spermatozoa to SIF pre-incubated with E-SBR, an ameliorative effect against SIF-induced impairment of sperm function could be observed through analysis of normal sperm parameters (motility, viability, morphology, Mg2+-dependent ATPase levels). E-SBR also blocked binding of labelled SIF to spermatozoa and bacteria and alleviated SIF-induced infertility in female BALB/c mice. This provided evidence for molecular similarities between bacteria and spermatozoa, owing to which anti-bacterial antibodies cross-reacting with spermatozoa might be produced and infertility might follow.
Additional keywords: mimicry, antisperm antibodies.
References
Boyce, A., Casey, A., and Walsh, G. (2004). A phytase enzyme-based biochemistry practical particularly suited to students undertaking courses in biotechnology and environmental science. Biochem. Mol. Biol. Educ. 32, 336–340.| A phytase enzyme-based biochemistry practical particularly suited to students undertaking courses in biotechnology and environmental science.Crossref | GoogleScholarGoogle Scholar |
Chappell, J. B. (1963). The effect of alkylguanidines on mitochondrial metabolism. J. Biol. Chem. 238, 410–417.
Deyhoul, N., Mohamaddoost, T., and Hosseini, M. (2017). Infertility-related risk factors: a systematic review. IJWHRS 5, 24–29.
| Infertility-related risk factors: a systematic review.Crossref | GoogleScholarGoogle Scholar |
Di Spiezio Sardo, A., Di Carlo, C., Minozzi, S., Spinelli, M., Pistotti, V., Alviggi, C., De Placido, G., Nappi, C., and Bifulco, G. (2016). Efficacy of hysteroscopy in improving reproductive outcomes of infertile couples: a systematic review and meta-analysis. Hum. Reprod. Update 22, 479–496.
| Efficacy of hysteroscopy in improving reproductive outcomes of infertile couples: a systematic review and meta-analysis.Crossref | GoogleScholarGoogle Scholar |
Eggert-Kruse, W., Huber, K., Rohr, G., and Runnebaum, B. (1993). Determination of antisperm antibodies in serum samples by means of enzyme-linked immunosorbent assay – a procedure to be recommended during infertility investigation? Hum. Reprod. 8, 1405–1413.
| Determination of antisperm antibodies in serum samples by means of enzyme-linked immunosorbent assay – a procedure to be recommended during infertility investigation?Crossref | GoogleScholarGoogle Scholar |
Eggert-Kruse, W., Buhlinger-Gopfarth, N., Probst, S., Aufenanger, J., Naher, H., and Runnebaum, B. (1996). Antibodies to Chlamydia trachomatis in semen and relationship with parameters of male fertility. Hum. Reprod. 11, 1408–1417.
| Antibodies to Chlamydia trachomatis in semen and relationship with parameters of male fertility.Crossref | GoogleScholarGoogle Scholar |
Emmens, C. W. (1947). The motility and viability of rabbit spermatozoa at different hydrogen ion concentrations. J. Physiol. 106, 471–481.
| The motility and viability of rabbit spermatozoa at different hydrogen ion concentrations.Crossref | GoogleScholarGoogle Scholar |
Figura, N., Piomboni, P., Ponzetto, A., Gambera, L., Lenzi, C., Vaira, D., Peris, C., Lotano, M. R., Gennari, L., Bianciardi, L., Renieri, T., Valensin, P. E., Capitani, S., Moretti, E., Colapinto, R., Baccetti, B., and Gennari, C. (2002). Helicobacter pylori infection and infertility. Eur. J. Gastroenterol. Hepatol. 14, 663–669.
| Helicobacter pylori infection and infertility.Crossref | GoogleScholarGoogle Scholar |
Hafez, E. S. E., and Kanagawa, H. (1973). Scanning electron microscopy of human, monkey and rabbit spermatozoa. Fertil. Steril. 24, 776–787.
| Scanning electron microscopy of human, monkey and rabbit spermatozoa.Crossref | GoogleScholarGoogle Scholar |
Kielley, M. W. (1955). Mitochondrial ATPase. In ‘Methods in Enzymology’. (Eds S. P. Colowick and N. O. Kaplan.) pp. 593–595. (Academic Press: New York.)
Kurpisz, M., and Alexander, N. J. (1995). Carbohydrate moieties on sperm surface: physiological relevance. Fertil. Steril. 63, 158–165.
| Carbohydrate moieties on sperm surface: physiological relevance.Crossref | GoogleScholarGoogle Scholar |
Kurpisz, M., Clark, G. F., Mahony, M., Anderson, T. L., and Alexander, N. J. (1989). Mouse monoclonal antibodies against human sperm: evidence for immunodominant glycosylated antigenic sites. Clin. Exp. Immunol. 78, 250–255.
Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 227, 680–685.
| Cleavage of structural proteins during assembly of head of bacteriophage T4.Crossref | GoogleScholarGoogle Scholar |
Lenzi, A., Gandini, L., Lombardo, F., Cappa, M., Nardini, P., Ferro, F., Borrelli, P., and Dondero, F. (1991). Antisperm antibodies in young boys. Andrologia 23, 233–235.
| Antisperm antibodies in young boys.Crossref | GoogleScholarGoogle Scholar |
Mahdi, B. M., Salih, W. H., Caitano, A. E., Kadhum, B. M., and Ibrahi, D. S. (2011). Frequency of antisperm antibodies in infertile women. J. Reprod. Infertil. 12, 261–265.
Sander, F. V., and Cramer, S. D. (1941). A practical method for testing the spermicidal action of chemical contraceptives. Hum. Fertil. 6, 134–137.
Shi, J., Yang, Z., Wang, M., Cheng, G., Li, D., Wang, Y., Zhou, Y., Liu, X., and Xu, C. (2007). Screening of an antigen target for immunocontraceptives from cross-reactive antigens between human sperm and Ureaplasma urealyticum. Infect. Immun. 75, 2004–2011.
| Screening of an antigen target for immunocontraceptives from cross-reactive antigens between human sperm and Ureaplasma urealyticum.Crossref | GoogleScholarGoogle Scholar |
Vander, H., Gupta, S., Kaur, S., Kaur, K., and Prabha, V. (2012). Characterization of sperm immobilization factor from Escherichia coli and its receptor to study the underlying mechanism of sperm immobilization. Am. J. Biomed. Sci. , .
| Characterization of sperm immobilization factor from Escherichia coli and its receptor to study the underlying mechanism of sperm immobilization.Crossref | GoogleScholarGoogle Scholar |
Witkin, S. S., Neuer, A., Giraldo, P., Jeremias, J., Tolbert, V., Korneeva, I. L., Kneissl, D., and Bongiovanni, A. M. (1997). Chlamydia trachomatis infection, immunity and pregnancy outcome. Infect. Dis. Obstet. Gynecol. 5, 128–132.
| Chlamydia trachomatis infection, immunity and pregnancy outcome.Crossref | GoogleScholarGoogle Scholar |
World Health Organization (WHO) (2010) ‘Laboratory manual for the examination and processing of human semen.’ (WHO Press: Geneva, Switzerland.)
