Comparative serum proteome analysis reveals potential early pregnancy-specific protein biomarkers in pigs
Ankan De A , Mohammad Ayub Ali A , Tukheswar Chutia B , Suneel Kumar Onteru C , Parthasarathi Behera A , Girin Kalita D , Sudarshan Kumar E and Jagan Mohanarao Gali
A F
+ Author Affiliations
- Author Affiliations
A Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, 796014, India.
B Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and A.H., Central Agricultural University, Selesih, Aizawl, Mizoram, 796014, India.
C Animal Biochemistry Division, Indian Council of Agricultural Research – National Dairy Research Institute, Karnal, Haryana, 132001, India.
D Department of Livestock Production Management, College of Veterinary Sciences and A.H., Central Agricultural University, Selesih, Aizawl, Mizoram, 796014, India.
E Animal Biotechnology Centre, Indian Council of Agricultural Research – National Dairy Research Institute, Karnal, Haryana, 132001, India.
F Corresponding author. Email: biochemjagan@gmail.com
Reproduction, Fertility and Development 31(3) 613-631 https://doi.org/10.1071/RD18227
Submitted: 16 June 2018 Accepted: 18 September 2018 Published: 13 November 2018
Abstract
In this study, the comparative serum proteome profile of Day 5, 12 and 16 of gestation, representing three early embryonic events, namely formation, elongation and implantation of blastocysts, and non-pregnant control were explored by a label-free quantitation-based mass spectrometric approach to identify early pregnancy biomarkers in pigs. A total of 131 proteins were identified with respect to different groups, out of which 105 were found to be differentially expressed proteins (DEPs). Among the DEPs, 54 and 66 proteins were found to be up and downregulated respectively in early pregnancy groups (fold change >2) and the maximum number of upregulated proteins was observed in the Day 12 pregnancy stage. Functional classification and pathway analysis of the DEPs revealed involvement of most of the proteins in complement and coagulation cascades, metabolic processes and immune and inflammatory responses. Proteins such as glutathione peroxidise (GPX), pregnancy zone protein (PZP), thrombospondin-1 (THBS1), α-1-antitrypsin (AAT) and mannose-binding lectin C (MBLC) were differentially expressed during early pregnancy and actively involved in different pregnancy-related activities. To the best of our knowledge, this is the first report on comparative serum protein profiling of different early pregnancy stages in pigs and our results provide a set of proteins that can be used as potential biomarkers for early pregnancy diagnosis in pigs.
Additional keywords : differentially expressed proteins, label-free, mass spectrometry.
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