Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content
Kelly Annes A B , Diego B. Müller A , Jorge A. P. Vilela A , Roniele S. Valente A , Diana P. Caetano A , Francielli W. S. Cibin A , Marcella P. Milazzotto B , Fernando S. Mesquita A , Katia R. A. Belaz C D , Marcos N. Eberlin C and Mateus J. Sudano
A B E
+ Author Affiliations
- Author Affiliations
A School of Veterinary Medicine, Federal University of Pampa, BR 472 – KM 592, PO Box 118, 97501-970, Uruguaiana, RS, Brazil.
B Centre of Natural and Human Sciences, Federal University of ABC, Av. dos Estados, 5001, 09210-580, Santo André, São Paulo, Brazil.
C ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, UNICAMP PO Box 6154, 13083–970, Distrito de Barão Geraldo, Campinas, São Paulo, Brazil.
D Institute of Chemistry, Federal University of Uberlândia (UFU), 38408-144, Uberlândia, Minas Gerais, Brazil.
E Corresponding author. Email: mjsudano@gmail.com
Reproduction, Fertility and Development 31(3) 462-472 https://doi.org/10.1071/RD18109
Abstract
This study assessed the lipid composition of oocytes from different follicle sizes and compared the expression of lipid-related genes and follicular fluid (FF) molecules between groups. We also investigated the functional consequences of differences on embryo development and blastocyst lipid deposits. Oocytes and FF were recovered from different follicle sizes. Oocytes from small (≤5 mm) and large (≥6mm) bovine follicles were used to produce Day 7 expanded blastocysts (Day7Ex) and blastocysts that only became expanded at Day 8 (Day8Ex) after insemination. Oocytes from >8 mm follicles had the highest lipid content. Few oocyte phospholipid variations were identified between groups. Very long chain fatty acid elongase 6 (ELOVL6) mRNA abundance was reduced in larger follicle-derived oocytes compared with the ≤2mm group. Increased levels of glucose, reactive oxygen species, glutathione and superoxide dismutase activity were also identified in FF from larger follicles. Large follicle-derived embryo development and lipid content of Day7Ex were greater than those derived from small follicles. Day8Ex had greater lipid deposition than Day7Ex. Oocytes and blastocysts exhibited follicle size-specific lipids. Large-follicle oocytes had increased lipid content and became Day7Ex with greater lipid deposition whereas delayed blastocoel expansion associated with a prolonged period of culture determined the lipid accumulation of Day8Ex. The FF microenvironment of large follicles seems to favour embryo development.
Additional keywords: follicular fluid microenvironment, in vitro production, lipid metabolism, mammalian egg, mass spectrometry.
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