Short-term effect of FSH on gene expression in bovine granulosa cells in vitro
Anne-Laure Nivet A , Isabelle Dufort A , Isabelle Gilbert A and Marc-André Sirard A B
+ Author Affiliations
- Author Affiliations
A Centre de recherche en reproduction, développement et santé intergénérationnelle, Faculté des sciences de l’agriculture et de l’alimentation, Département des sciences animales, Université Laval, Québec, QC G1V 0A6, Canada.
B Corresponding author. Email: marc-andre.sirard@fsaa.ulaval.ca
Reproduction, Fertility and Development 30(8) 1154-1160 https://doi.org/10.1071/RD17469
Submitted: 27 June 2017 Accepted: 25 January 2018 Published: 13 March 2018
Abstract
In reproduction, FSH is one of the most important hormones, especially in females, because it controls the number of follicles and the rate of follicular growth. Although several studies have examined the follicular response at the transcriptome level, it is difficult to obtain a clear and complete picture of the genes responding to an increase in FSH in an in vivo context because follicles undergo rapid morphological and physical changes during their growth. To help define the transcriptome downstream response to FSH, an in vitro model was used in the present study to observe the short-term (4 h) cellular response. Gene expression analysis highlighted a set of novel transcripts that had not been reported previously as being part of the FSH response. Moreover, the results of the present study indicate that the epithelial to mesenchymal transition pathway is inhibited by short-term FSH stimuli, maintaining follicles in a growth phase and preventing differentiation. Modulating gene expression in vitro has physiological limitations, but it can help assess the potential downstream response and begin the mapping of the granulosa cell transcriptome in relation to FSH. This information is a key feature to help discriminate between the effects of FSH and LH, or to elucidate the overlapping of insulin-like growth factor 1 and FSH in the granulosa mitogenic response.
Additional keywords: cow, follicle, in-vitro culture, ovary.
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