Metabolomic profiling of bovine oviductal fluid across the oestrous cycle using proton nuclear magnetic resonance spectroscopy
Julie Lamy A , Julie Gatien B , Florine Dubuisson A , Lydie Nadal-Desbarats C , Pascal Salvetti B , Pascal Mermillod A and Marie Saint-Dizier A C D
+ Author Affiliations
- Author Affiliations
A UMR85 PRC, INRA, CNRS, IFCE, Université de Tours, 37380 Nouzilly, France.
B ALLICE, 37380 Nouzilly, France.
C Université de Tours, 37000 Tours, France.
D Corresponding author. Email: marie.saint-dizier@univ-tours.fr
Reproduction, Fertility and Development 30(7) 1021-1028 https://doi.org/10.1071/RD17389
Submitted: 26 September 2017 Accepted: 19 November 2017 Published: 5 January 2018
Abstract
In the present study we tested whether regulation of the metabolome in bovine oviductal fluid depended on the stage of the oestrous cycle, the side relative to ovulation and local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected in the preovulatory, postovulatory, mid- and late luteal phases, from cyclic cows at a local abattoir (18–27 cows per stage and side). The metabolomes were assessed by proton nuclear magnetic resonance spectroscopy (H-NMR). In all, 39 metabolites were identified, among which the amino acid glycine and the energy substrates lactate and myoinositol were the most abundant at all stages. The concentrations of 14 metabolites varied according to the stage of the oestrous cycle in at least one side relative to ovulation, of which four (choline, glucose-1-phosphate, glycine and pyruvate) were correlated with intraoviductal progesterone or oestradiol concentrations. Glucose-1-phosphate was most affected by the stage of the cycle, with four- to sixfold higher levels in luteal than periovulatory stages. These results provide new knowledge on the regulation of secretory activity in the oviduct and may help optimise culture media for gamete maturation, IVF and embryo production.
Additional keywords: 1H-NMR, metabolome, steroid hormones, tubal fluid.
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