Extracellular-like matrices and leukaemia inhibitory factor for in vitro culture of human primordial follicles
Assiel J. Younis A B , Galit Lerer-Serfaty A B , Dana Stav A B , Bethsabee Sabbah A B F , Tzippy Shochat C , Gania Kessler-Icekson B D , Muayad A. Zahalka B D , Michal Shachar-Goldenberg E , Avi Ben-Haroush A B , Benjamin Fisch A B and Ronit Abir A B G
+ Author Affiliations
- Author Affiliations
A Infertility and IVF Unit, Beilinson Women Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
B Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, PO Box 39040, Tel Aviv, Israel.
C Statistical Consulting Unit, Beilinson Hospital, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
D The Felsenstein Medical Research Center, Rabin Medical Center, 39 Jabotinski Street, Petach Tikva 49100, Israel.
E Sami Shamoon College of Engineering, PO Box 950, Beer-Sheva 84100, Israel.
F Present address: Department of Pediatrics, Jacobi Medical Center, Bronx, NY 10461, USA.
G Corresponding author. Email: ronita@clalit.org.il
Reproduction, Fertility and Development 29(10) 1982-1994 https://doi.org/10.1071/RD16233
Submitted: 5 June 2016 Accepted: 24 November 2016 Published: 1 February 2017
Abstract
The possibility of maturing human primordial follicles in vitro would assist fertility restoration without the danger of reseeding malignancies. Leukaemia inhibitory factor (LIF) and certain culture matrices may promote human follicular growth. The present study compared human primordial follicular growth on novel culture matrices, namely human recombinant vitronectin (hrVit), small intestine submucosa (SIS), alginate scaffolds and human recombinant virgin collagen bioengineered in tobacco plant lines (CollPlant). The frozen–thawed ovarian samples that were used had been obtained from girls or young women undergoing fertility preservation. In the first part of the study, 20 samples were cultured for 6 days on hrVit or SIS with basic culture medium alone or supplemented with one of two concentrations of LIF (10 ng mL–1 and 100 ng mL–1), with and without LIF-neutralising antibody. In the second part of the study, 15 samples were cultured for 6 days on alginate scaffolds or CollPlant matrices with basic culture medium. Follicular development was assessed by follicular counts and classification, Ki67 immunohistochemistry and 17β-oestradiol and anti-Müllerian hormone measurements in spent media samples. Primordial follicular growth was not enhanced by LIF. Despite some significant differences among the four matrices, none appeared to have a clear advantage, apart from significantly more Ki67-stained follicles on alginate and CollPlant matrices. Further studies of other culture matrices and medium supplements are needed to obtain an optimal system.
Additional keywords: alginate scaffolds, anti-Müllerian hormone (AMH), CollPlant matrices, human recombinant vitronectin (hrVit), 17β-oestradiol, small intestinal submucosa (SIS).
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