Putrescine supplementation during in vitro maturation of aged mouse oocytes improves the quality of blastocysts
Dandan Liu A B C F , Guolong Mo A D , Yong Tao A , Hongmei Wang B C G and X. Johné Liu A D E G
+ Author Affiliations
- Author Affiliations
A Ottawa Hospital Research Institute, The Ottawa Hospital – General Campus, 501 Smyth Road, Box 511, Ottawa, ON, K1H 8L6, Canada.
B State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
C University of Chinese Academy of Sciences, Beijing, 100049, China.
D Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, K1H 8L6, Canada.
E Department of Obstetrics and Gynaecology, University of Ottawa, Ottawa, ON, K1H 8L6, Canada.
F Present address: Centre of Reproductive Medicine, Department of Obstetrics and Gynaecology, Peking University Third Hospital, 49 North Huayuan Road, Haidian District, Beijing, 100191, China.
G Corresponding authors. Emails: wanghm@ioz.ac.cn; jliu@ohri.ca
Reproduction, Fertility and Development 29(7) 1392-1400 https://doi.org/10.1071/RD16061
Submitted: 5 February 2016 Accepted: 12 May 2016 Published: 20 June 2016
Abstract
Mouse ovaries exhibit a peri-ovulatory rise of ornithine decarboxylase and its product putrescine concurrent with oocyte maturation. Older mice exhibit a deficiency of both the enzyme and putrescine. Peri-ovulatory putrescine supplementation in drinking water increases ovarian putrescine levels, reduces embryo resorption and increases live pups in older mice. However, it is unknown if putrescine acts in the ovaries to improve oocyte maturation. This study examined the impact of putrescine supplementation during oocyte in vitro maturation (IVM) on the developmental potential of aged oocytes. Cumulus–oocyte complexes from 9–12-month-old C57BL/6 mice were subjected to IVM with or without 0.5 mM putrescine, followed by in vitro fertilisation and culture to the blastocyst stage. Putrescine supplementation during IVM did not influence the proportion of oocyte maturation, fertilisation or blastocyst formation, but significantly increased blastocyst cell numbers (44.5 ± 1.9, compared with 36.5 ± 1.9 for control; P = 0.003). The putrescine group also had a significantly higher proportion of blastocysts with top-grade morphology (42.9%, compared with 26.1% for control; P = 0.041) and a greater proportion with octamer-binding transcription factor 4 (OCT4)-positive inner cell mass (38.3%, compared with 19.8% for control; P = 0.005). Therefore, putrescine supplementation during IVM improves egg quality of aged mice, providing proof of principle for possible application in human IVM procedures for older infertile women.
Additional keywords: aging, inner cell mass, in vitro fertilisation (IVF).
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