Preantral follicle density in ovarian biopsy fragments and effects of mare age
K. A. Alves A B , B. G. Alves A , G. D. A. Gastal A , K. T. Haag A , M. O. Gastal A , J. R. Figueiredo C , M. L. Gambarini B and E. L. Gastal A D
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, Food and Nutrition, Southern Illinois University, 1205 Lincoln Dr, Carbondale, IL 62901, USA.
B Center for Studies and Research in Animal Reproductive Biology, College of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
C Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Campus do Itaperi, 60714-903, Fortaleza, CE, Brazil.
D Corresponding author. Email: egastal@siu.edu
Reproduction, Fertility and Development 29(5) 867-875 https://doi.org/10.1071/RD15402
Submitted: 21 June 2015 Accepted: 14 December 2015 Published: 5 February 2016
Abstract
The aims of the present study were to: (1) evaluate preantral follicle density in ovarian biopsy fragments within and among mares; (2) assess the effects of mare age on the density and quality of preantral follicles; and (3) determine the minimum number of ovarian fragments and histological sections needed to estimate equine follicle density using a mathematical model. The ovarian biopsy pick-up method was used in three groups of mares separated according to age (5–6, 7–10 and 11–16 years). Overall, 336 preantral follicles were recorded with a mean follicle density of 3.7 follicles per cm2. Follicle density differed (P < 0.05) among animals, ovarian fragments from the same animal, histological sections and age groups. More (P < 0.05) normal follicles were observed in the 5–6 years (97%) than the 11–16 years (84%) age group. Monte Carlo simulations showed a higher probability (90%; P < 0.05) of detecting follicle density using two experimental designs with 65 histological sections and three to four ovarian fragments. In summary, equine follicle density differed among animals and within ovarian fragments from the same animal, and follicle density and morphology were negatively affected by aging. Moreover, three to four ovarian fragments with 65 histological sections were required to accurately estimate follicle density in equine ovarian biopsy fragments.
Additional keywords: aging, female, fertility preservation, horse, ovary.
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