Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro
Ricaurte Lopera-Vasquez A , Meriem Hamdi A , Veronica Maillo A , Valeriano Lloreda A , Pilar Coy B , Alfonso Gutierrez-Adan A , Pablo Bermejo-Alvarez A and Dimitrios Rizos A C
+ Author Affiliations
- Author Affiliations
A Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de la Coruna Km 5.9, Madrid, 28040, Spain.
B Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Murcia, 30071, Spain.
C Corresponding author. Email: drizos@inia.es
Reproduction, Fertility and Development 29(3) 621-629 https://doi.org/10.1071/RD15238
Submitted: 12 June 2015 Accepted: 2 September 2015 Published: 14 October 2015
Abstract
To evaluate the effect of bovine oviductal fluid (OF) supplementation during in vitro culture of bovine embryos on their development and quality, in vitro-produced zygotes were cultured in synthetic oviductal fluid (SOF; negative control; C–) supplemented with OF or 5% fetal calf serum (positive control; C+). Embryo development was recorded on Days 7–9 after insemination and blastocyst quality was assessed through cryotolerance, differential cell counting of the inner cell mass and trophectoderm, and gene expression. OF was added to the culture medium at concentrations ranging from 0.625% to 25%. The higher OF concentrations (5%, 10% and 25%) had a detrimental effect on embryo development. Lower OF concentrations (1.25% and 0.625%) supported embryo development until Day 9 (27.5%) and produced higher-quality blastocysts, as reflected by their cryotolerance (53.6% and 57.7% survival at 72 h, respectively, vs 25.9% in C+) and total cell number (mean (± s.e.m.) 165.1 ± 4.7 and 156.2 ± 4.2, respectively, vs 127.7 ± 4.9 in C– and 143.1 ± 4.9 in C+). Consistent with these data, upregulation of the water channel aquaporin 3 (AQP3) mRNA was observed in blastocysts supplemented with 1.25% OF compared with C– and C+. Serum supplementation resulted in a reduction in the expression of glucose and lipid metabolism-related genes and downregulation of the epigenetic-related genes DNA methyltransferase 3A (DNMT3A) and insulin-like growth factor 2 receptor (IGF2R). In conclusion, in vitro culture with low concentrations of OF has a positive effect on the development and quality of bovine embryos.
Additional keywords: embryo culture, gene expression.
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