Morphometric analysis and developmental comparison of embryos from carriers with balanced chromosomal rearrangements in preimplantation genetic diagnosis cycles
Baoheng Gui A , Zhongyuan Yao A , Yanru Huang A , Libin Mei A , Yanping Li B , Donge Liu B , Nenghui Liu B , Yan Xia A , Desheng Liang A and Lingqian Wu A C
+ Author Affiliations
- Author Affiliations
A The State Key Laboratory of Medical Genetics of China, Central South University, No. 110, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
B The Reproductive Medical Center of Xiangya Hospital, Central South University, No. 87, Xiangya Rd, Changsha, Hunan, 410008, P. R. China.
C Corresponding author. Email: wulingqian@sklmg.edu.cn
Reproduction, Fertility and Development 28(12) 1953-1963 https://doi.org/10.1071/RD15093
Submitted: 9 March 2015 Accepted: 28 May 2015 Published: 29 June 2015
Abstract
The morphological parameters of embryos from 22 carriers with balanced chromosomal rearrangements (CRs) were quantified and evaluated to determine their possible link to chromosomal composition. The morphometric characteristics of 168 embryos diagnosed by fluorescence in situ hybridisation were measured using an imaging tool and then analysed retrospectively. The mean zygotic diameter of normal–balanced embryos was significantly smaller compared with that of abnormal embryos (P = 0.015). In addition, the reduction in total cytoplasmic volume for Day-3 embryos was significantly lower in normal or balanced embryos than in abnormal embryos (P = 0.027). Moreover, the pronuclear volumes of embryos that failed to reach the blastocyst stage were significantly smaller compared with those of blastocysts (P = 0.016). These findings indicate that morphometric characteristics are correlated with developmental outcomes as well as with chromosomal composition in embryos from balanced CR carriers. However, an effective indicator of developmental outcomes may not accurately reflect chromosomal composition. Combining morphometric and traditional qualitative assessment may increase the precision and standardisation of embryo evaluation as well as contributing to improved efficiency of preimplantation genetic diagnosis by selecting embryos with high developmental potential and preferentially testing embryos predicted to have a low risk of chromosomal imbalance.
Additional keywords: chromosomal imbalance, diameter, embryo assessment, embryo development, volume.
References
Acloque, H., Bonnet-Garnier, A., Mompart, F., Pinton, A., and Yerle-Bouissou, M. (2013). Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17). PLoS One 8, e78005.| Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsleqt7zJ&md5=efaf22eca5ec64d0e0f90d2e4f3736bfCAS | 24205066PubMed |
Adenot, P. G., Mercier, Y., Renard, J. P., and Thompson, E. M. (1997). Differential H4 acetylation of paternal and maternal chromatin precedes DNA replication and differential transcriptional activity in pronuclei of one-cell mouse embryos. Development 124, 4615–4625.
| 1:CAS:528:DyaK1cXht12jsA%3D%3D&md5=5202c4d0f3d3117efa41c52e72e02dc3CAS | 9409678PubMed |
Agerholm, I. E., Hnida, C., Cruger, D. G., Berg, C., Bruun-Petersen, G., Kolvraa, S., and Ziebe, S. (2008). Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four-cell embryos. J. Assist. Reprod. Genet. 25, 95–102.
| Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four-cell embryos.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1c7lt1GqsQ%3D%3D&md5=b611287ad4ed5f5107d54f1dd8bb5be4CAS | 18256921PubMed |
Alfarawati, S., Fragouli, E., Colls, P., Stevens, J., Gutierrez-Mateo, C., Schoolcraft, W. B., Katz-Jaffe, M. G., and Wells, D. (2011). The relationship between blastocyst morphology, chromosomal abnormality and embryo gender. Fertil. Steril. 95, 520–524.
| The relationship between blastocyst morphology, chromosomal abnormality and embryo gender.Crossref | GoogleScholarGoogle Scholar | 20537630PubMed |
Alfarawati, S., Fragouli, E., Colls, P., and Wells, D. (2012). Embryos of Robertsonian translocation carriers exhibit a mitotic interchromosomal effect that enhances genetic instability during early development. PLoS Genet. 8, e1003025.
| Embryos of Robertsonian translocation carriers exhibit a mitotic interchromosomal effect that enhances genetic instability during early development.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhs1Ois7jK&md5=e873c42a94054949d54c06f8000293e7CAS | 23133396PubMed | 23133396PubMed |
Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group Embryology (2011a). Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Reprod. Biomed. Online 22, 632–646.
| 21481639PubMed |
Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology (2011b). The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum. Reprod. 26, 1270–1283.
| The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting.Crossref | GoogleScholarGoogle Scholar | 21502182PubMed | 21502182PubMed |
Arroyo, G., Veiga, A., Santalo, J., and Barri, P. N. (2007). Developmental prognosis for zygotes based on pronuclear pattern: usefulness of pronuclear scoring. J. Assist. Reprod. Genet. 24, 173–181.
| Developmental prognosis for zygotes based on pronuclear pattern: usefulness of pronuclear scoring.Crossref | GoogleScholarGoogle Scholar | 17318392PubMed | 17318392PubMed |
Basile, N., Nogales Mdel, C., Bronet, F., Florensa, M., Riqueiros, M., Rodrigo, L., Garcia-Velasco, J., and Meseguer, M. (2014). Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis. Fertil. Steril. 101, 699–704.e1.
| Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis.Crossref | GoogleScholarGoogle Scholar | 24424365PubMed | 24424365PubMed |
Benkhalifa, M., Janny, L., Vye, P., Malet, P., Boucher, D., and Menezo, Y. (1993). Assessment of polyploidy in human morulae and blastocysts using co-culture and fluorescent in situ hybridisation. Hum. Reprod. 8, 895–902.
| 1:STN:280:DyaK3szks1KjsA%3D%3D&md5=cbc1fc4a20c61e31df481c3889f928d0CAS |
| 1:STN:280:DyaK3szks1KjsA%3D%3D&md5=cbc1fc4a20c61e31df481c3889f928d0CAS | 8345081PubMed | 8345081PubMed |
Beuchat, A., Thevenaz, P., Unser, M., Ebner, T., Senn, A., Urner, F., Germond, M., and Sorzano, C. O. (2008). Quantitative morphometrical characterisation of human pronuclear zygotes. Hum. Reprod. 23, 1983–1992.
| Quantitative morphometrical characterisation of human pronuclear zygotes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1crisFaitg%3D%3D&md5=e90d38513935e9f58698257e94f4a479CAS | 1:STN:280:DC%2BD1crisFaitg%3D%3D&md5=e90d38513935e9f58698257e94f4a479CAS | 18540007PubMed | 18540007PubMed |
Bint, S. M., Ogilvie, C. M., Flinter, F. A., Khalaf, Y., and Scriven, P. N. (2011). Meiotic segregation of Robertsonian translocations ascertained in cleavage-stage embryos – implications for preimplantation genetic diagnosis. Hum. Reprod. 26, 1575–1584.
| Meiotic segregation of Robertsonian translocations ascertained in cleavage-stage embryos – implications for preimplantation genetic diagnosis.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MrisV2gtw%3D%3D&md5=eacd7163a10c04fa2e84ec8c8163510aCAS | 1:STN:280:DC%2BC3MrisV2gtw%3D%3D&md5=eacd7163a10c04fa2e84ec8c8163510aCAS | 21441546PubMed | 21441546PubMed |
Braga, D. P., Setti, A. S., Figueira Rde, C., Iaconelli, A., and Borges, E. (2013). The combination of pronuclear and blastocyst morphology: a strong prognostic tool for implantation potential. J. Assist. Reprod. Genet. 30, 1327–1332.
| The combination of pronuclear and blastocyst morphology: a strong prognostic tool for implantation potential.Crossref | GoogleScholarGoogle Scholar | 23934020PubMed | 23934020PubMed |
Campana, M., Serra, A., and Neri, G. (1986). Role of chromosome aberrations in recurrent abortion: a study of 269 balanced translocations. Am. J. Med. Genet. 24, 341–356.
| Role of chromosome aberrations in recurrent abortion: a study of 269 balanced translocations.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL283jvFensA%3D%3D&md5=c42e4b70a1d937ac18ff37c348b988c2CAS | 1:STN:280:DyaL283jvFensA%3D%3D&md5=c42e4b70a1d937ac18ff37c348b988c2CAS | 3717213PubMed | 3717213PubMed |
Campbell, A., Fishel, S., Bowman, N., Duffy, S., Sedler, M., and Hickman, C. F. (2013). Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod. Biomed. Online 26, 477–485.
| Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics.Crossref | GoogleScholarGoogle Scholar | 23518033PubMed | 23518033PubMed |
Carrera, M., and Veiga, A. (1998). Chromosome analysis of human preimplantation embryos. Croat. Med. J. 39, 121–127.
| 1:STN:280:DyaK1c3ksFWktw%3D%3D&md5=50f6215fb4bf65bfe015d148e754bb84CAS |
| 1:STN:280:DyaK1c3ksFWktw%3D%3D&md5=50f6215fb4bf65bfe015d148e754bb84CAS | 9575266PubMed | 9575266PubMed |
Chavez, S. L., Loewke, K. E., Han, J., Moussavi, F., Colls, P., Munne, S., Behr, B., and Reijo Pera, R. A. (2012). Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage. Nat. Commun. 3, 1251.
| Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage.Crossref | GoogleScholarGoogle Scholar | 23212380PubMed | 23212380PubMed |
Conn, C. M., Harper, J. C., Winston, R. M., and Delhanty, J. D. (1998). Infertile couples with Robertsonian translocations: preimplantation genetic analysis of embryos reveals chaotic cleavage divisions. Hum. Genet. 102, 117–123.
| Infertile couples with Robertsonian translocations: preimplantation genetic analysis of embryos reveals chaotic cleavage divisions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjtlSntQ%3D%3D&md5=61ec72a031cbc106eace3037c13f9edeCAS | 1:CAS:528:DyaK1cXjtlSntQ%3D%3D&md5=61ec72a031cbc106eace3037c13f9edeCAS | 9490289PubMed | 9490289PubMed |
De Braekeleer, M., and Dao, T. N. (1991). Cytogenetic studies in male infertility: a review. Hum. Reprod. 6, 245–250.
| 1:STN:280:DyaK3M3nslWntQ%3D%3D&md5=0fae5ede597cc292e7f4ac0ea011a276CAS |
| 1:STN:280:DyaK3M3nslWntQ%3D%3D&md5=0fae5ede597cc292e7f4ac0ea011a276CAS | 2056021PubMed | 2056021PubMed |
Eaker, S., Pyle, A., Cobb, J., and Handel, M. A. (2001). Evidence for meiotic spindle checkpoint from analysis of spermatocytes from Robertsonian-chromosome heterozygous mice. J. Cell Sci. 114, 2953–2965.
| 1:CAS:528:DC%2BD3MXmvFCqsLo%3D&md5=374fe77a7e0e6f515f4437c861b8cfbeCAS |
| 1:CAS:528:DC%2BD3MXmvFCqsLo%3D&md5=374fe77a7e0e6f515f4437c861b8cfbeCAS | 11686299PubMed | 11686299PubMed |
Eaton, J. L., Hacker, M. R., Harris, D., Thornton, K. L., and Penzias, A. S. (2009). Assessment of Day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage. Fertil. Steril. 91, 2432–2436.
| Assessment of Day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage.Crossref | GoogleScholarGoogle Scholar | 18440522PubMed | 18440522PubMed |
Encalada, S. E., Willis, J., Lyczak, R., and Bowerman, B. (2005). A spindle checkpoint functions during mitosis in the early Caenorhabditis elegans embryo. Mol. Biol. Cell 16, 1056–1070.
| A spindle checkpoint functions during mitosis in the early Caenorhabditis elegans embryo.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXit1Srsbo%3D&md5=7dfffec092154253370389aa143389d5CAS | 1:CAS:528:DC%2BD2MXit1Srsbo%3D&md5=7dfffec092154253370389aa143389d5CAS | 15616189PubMed | 15616189PubMed |
Evsikov, S., and Verlinsky, Y. (1998). Mosaicism in the inner cell mass of human blastocysts. Hum. Reprod. 13, 3151–3155.
| Mosaicism in the inner cell mass of human blastocysts.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1M%2FmvVOlsA%3D%3D&md5=b6f65686fcbfae7c6cef27557904212cCAS | 1:STN:280:DyaK1M%2FmvVOlsA%3D%3D&md5=b6f65686fcbfae7c6cef27557904212cCAS | 9853873PubMed | 9853873PubMed |
Findikli, N., Kahraman, S., Kumtepe, Y., Donmez, E., Biricik, A., Sertyel, S., Berkil, H., and Melil, S. (2003). Embryo development characteristics in Robertsonian and reciprocal translocations: a comparison of results with non-translocation cases. Reprod. Biomed. Online 7, 563–571.
| Embryo development characteristics in Robertsonian and reciprocal translocations: a comparison of results with non-translocation cases.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3sngtVSmtA%3D%3D&md5=b37ac8ff1a06974474fc884e3833ce4aCAS | 1:STN:280:DC%2BD3sngtVSmtA%3D%3D&md5=b37ac8ff1a06974474fc884e3833ce4aCAS | 14680550PubMed | 14680550PubMed |
Fu, J., Wang, X. J., Wang, Y. W., Sun, J., Gemzell-Danielsson, K., and Sun, X. X. (2009). The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome. J. Assist. Reprod. Genet. 26, 437–441.
| The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFOhu7%2FO&md5=03f4681740183df29a5f4bbe7104994aCAS | 1:CAS:528:DC%2BD1MXhsFOhu7%2FO&md5=03f4681740183df29a5f4bbe7104994aCAS | 19789972PubMed | 19789972PubMed |
Gianaroli, L., Magli, M. C., Ferraretti, A. P., Munne, S., Balicchia, B., Escudero, T., and Crippa, A. (2002). Possible interchromosomal effect in embryos generated by gametes from translocation carriers. Hum. Reprod. 17, 3201–3207.
| Possible interchromosomal effect in embryos generated by gametes from translocation carriers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXltFamtg%3D%3D&md5=5ac9ba2464378c23985a0a1d70083449CAS | 1:CAS:528:DC%2BD3sXltFamtg%3D%3D&md5=5ac9ba2464378c23985a0a1d70083449CAS | 12456624PubMed | 12456624PubMed |
Hamerton, J. L., Ray, M., Abbott, J., Williamson, C., and Ducasse, G. C. (1972). Chromosome studies in a neonatal population. Can. Med. Assoc. J. 106, 776–779.
| 1:STN:280:DyaE387lsFentw%3D%3D&md5=51cf11cbd9bb8b23a2db87c44497a3a0CAS |
| 1:STN:280:DyaE387lsFentw%3D%3D&md5=51cf11cbd9bb8b23a2db87c44497a3a0CAS | 4259580PubMed | 4259580PubMed |
Hamerton, J. L., Canning, N., Ray, M., and Smith, S. (1975). A cytogenetic survey of 14069 newborn infants. I. Incidence of chromosome abnormalities. Clin. Genet. 8, 223–243.
| A cytogenetic survey of 14069 newborn infants. I. Incidence of chromosome abnormalities.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaE28%2Fkt1CitQ%3D%3D&md5=2a25146cf3e52f9a25af2649f1a2c1b6CAS | 1:STN:280:DyaE28%2Fkt1CitQ%3D%3D&md5=2a25146cf3e52f9a25af2649f1a2c1b6CAS | 1183067PubMed | 1183067PubMed |
Hardarson, T., Hanson, C., Sjogren, A., and Lundin, K. (2001). Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum. Reprod. 16, 313–318.
| Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M3ksV2htQ%3D%3D&md5=a9679c022e642b60734c37d3c3cecec1CAS | 1:STN:280:DC%2BD3M3ksV2htQ%3D%3D&md5=a9679c022e642b60734c37d3c3cecec1CAS | 11157826PubMed | 11157826PubMed |
Hardarson, T., Caisander, G., Sjogren, A., Hanson, C., Hamberger, L., and Lundin, K. (2003). A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts. Hum. Reprod. 18, 399–407.
| A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXit1yrtLw%3D&md5=36b926d0e240cde241b381cb251e07f4CAS | 12571180PubMed | 12571180PubMed |
Hnida, C., and Ziebe, S. (2004). Total cytoplasmic volume as biomarker of fragmentation in human embryos. J. Assist. Reprod. Genet. 21, 335–340.
| Total cytoplasmic volume as biomarker of fragmentation in human embryos.Crossref | GoogleScholarGoogle Scholar | 15587147PubMed | 15587147PubMed |
Hnida, C., Engenheiro, E., and Ziebe, S. (2004). Computer-controlled, multilevel, morphometric analysis of blastomere size as biomarker of fragmentation and multinuclearity in human embryos. Hum. Reprod. 19, 288–293.
| Computer-controlled, multilevel, morphometric analysis of blastomere size as biomarker of fragmentation and multinuclearity in human embryos.Crossref | GoogleScholarGoogle Scholar | 14747169PubMed | 14747169PubMed |
Hnida, C., Agerholm, I., and Ziebe, S. (2005). Traditional detection versus computer-controlled multilevel analysis of nuclear structures from donated human embryos. Hum. Reprod. 20, 665–671.
| Traditional detection versus computer-controlled multilevel analysis of nuclear structures from donated human embryos.Crossref | GoogleScholarGoogle Scholar | 15591086PubMed | 15591086PubMed |
Jacobs, P. A., Melville, M., Ratcliffe, S., Keay, A. J., and Syme, J. (1974). A cytogenetic survey of 11680 newborn infants. Ann. Hum. Genet. 37, 359–376.
| A cytogenetic survey of 11680 newborn infants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaE2M%2Fgs1ajsQ%3D%3D&md5=cb52befbd5ec9f3bbbaf89240bbdd4c8CAS | 1:STN:280:DyaE2M%2Fgs1ajsQ%3D%3D&md5=cb52befbd5ec9f3bbbaf89240bbdd4c8CAS | 4277977PubMed | 4277977PubMed |
Johansson, M., Hardarson, T., and Lundin, K. (2003). There is a cutoff limit in diameter between a blastomere and a small anucleate fragment. J. Assist. Reprod. Genet. 20, 309–313.
| There is a cutoff limit in diameter between a blastomere and a small anucleate fragment.Crossref | GoogleScholarGoogle Scholar | 12948092PubMed | 12948092PubMed |
Jones, E. L., Mudrak, O., and Zalensky, A. O. (2010). Kinetics of human male pronuclear development in a heterologous ICSI model. J. Assist. Reprod. Genet. 27, 277–283.
| Kinetics of human male pronuclear development in a heterologous ICSI model.Crossref | GoogleScholarGoogle Scholar | 20221683PubMed | 20221683PubMed |
Lejeune, J. (1963). Autosomal disorders. Pediatrics 32, 326–337.
| 1:STN:280:DyaF2c%2FitFWgug%3D%3D&md5=c0f7e3ac7a5b4cb1ac1562309a5fe802CAS |
| 1:STN:280:DyaF2c%2FitFWgug%3D%3D&md5=c0f7e3ac7a5b4cb1ac1562309a5fe802CAS | 14063510PubMed | 14063510PubMed |
Lundqvist, M., Johansson, U., Lundkvist, O., Milton, K., Westin, C., and Simberg, N. (2001). Does pronuclear morphology and/or early cleavage rate predict embryo implantation potential? Reprod. Biomed. Online 2, 12–16.
| Does pronuclear morphology and/or early cleavage rate predict embryo implantation potential?Crossref | GoogleScholarGoogle Scholar | 12537819PubMed | 12537819PubMed |
Magli, M. C., Gianaroli, L., Ferraretti, A. P., Lappi, M., Ruberti, A., and Farfalli, V. (2007). Embryo morphology and development are dependent on the chromosomal complement. Fertil. Steril. 87, 534–541.
| Embryo morphology and development are dependent on the chromosomal complement.Crossref | GoogleScholarGoogle Scholar | 17123520PubMed | 17123520PubMed |
Ménézo, Y. J., Bellec, V., Zaroukian, A., and Benkhalifa, M. (1997). Embryo selection by IVF, co-culture and transfer at the blastocyst stage in case of translocation. Hum. Reprod. 12, 2802–2803.
| Embryo selection by IVF, co-culture and transfer at the blastocyst stage in case of translocation.Crossref | GoogleScholarGoogle Scholar | 9455856PubMed | 9455856PubMed |
Ménézo, Y., Chouteau, J., and Veiga, A. (2001). In vitro fertilisation and blastocyst transfer for carriers of chromosomal translocation. Eur. J. Obstet. Gynecol. Reprod. Biol. 96, 193–195.
| In vitro fertilisation and blastocyst transfer for carriers of chromosomal translocation.Crossref | GoogleScholarGoogle Scholar | 11384806PubMed | 11384806PubMed |
Montag, M., Liebenthron, J., and Koster, M. (2011). Which morphological scoring system is relevant in human embryo development? Placenta 32, S252–S256.
| Which morphological scoring system is relevant in human embryo development?Crossref | GoogleScholarGoogle Scholar | 21782239PubMed | 21782239PubMed |
Munné, S. (2006). Chromosome abnormalities and their relationship to morphology and development of human embryos. Reprod. Biomed. Online 12, 234–253.
| Chromosome abnormalities and their relationship to morphology and development of human embryos.Crossref | GoogleScholarGoogle Scholar | 16478592PubMed | 16478592PubMed |
Munné, S., Alikani, M., Tomkin, G., Grifo, J., and Cohen, J. (1995). Embryo morphology, developmental rates and maternal age are correlated with chromosome abnormalities. Fertil. Steril. 64, 382–391.
| 7615118PubMed |
| 7615118PubMed |
Munné, S., Chen, S., Colls, P., Garrisi, J., Zheng, X., Cekleniak, N., Lenzi, M., Hughes, P., Fischer, J., Garrisi, M., Tomkin, G., and Cohen, J. (2007). Maternal age, morphology, development and chromosome abnormalities in over 6000 cleavage-stage embryos. Reprod. Biomed. Online 14, 628–634.
| Maternal age, morphology, development and chromosome abnormalities in over 6000 cleavage-stage embryos.Crossref | GoogleScholarGoogle Scholar | 17509208PubMed | 17509208PubMed |
Paternot, G., Debrock, S., D’Hooghe, T., and Spiessens, C. (2011). Computer-assisted embryo selection: a benefit in the evaluation of embryo quality? Reprod. Biomed. Online 23, 347–354.
| Computer-assisted embryo selection: a benefit in the evaluation of embryo quality?Crossref | GoogleScholarGoogle Scholar | 21782515PubMed | 21782515PubMed |
Paternot, G., Debrock, S., De Neubourg, D., D’Hooghe, T. M., and Spiessens, C. (2013). Semi-automated morphometric analysis of human embryos can reveal correlations between total embryo volume and clinical pregnancy. Hum. Reprod. 28, 627–633.
| Semi-automated morphometric analysis of human embryos can reveal correlations between total embryo volume and clinical pregnancy.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3s3ptFSntA%3D%3D&md5=bf376a82dd181beadade2fc7915fdb16CAS | 1:STN:280:DC%2BC3s3ptFSntA%3D%3D&md5=bf376a82dd181beadade2fc7915fdb16CAS | 23315063PubMed | 23315063PubMed |
Payne, D., Flaherty, S. P., Barry, M. F., and Matthews, C. D. (1997). Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum. Reprod. 12, 532–541.
| Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2s3nslSlsA%3D%3D&md5=57cbf71e440e445e9ed69873d1df81b8CAS | 1:STN:280:DyaK2s3nslSlsA%3D%3D&md5=57cbf71e440e445e9ed69873d1df81b8CAS | 9130755PubMed | 9130755PubMed |
Pellestor, F., Sele, B., Jalbert, H., and Jalbert, P. (1989). Direct segregation analysis of reciprocal translocations: a study of 283 sperm karyotypes from four carriers. Am. J. Hum. Genet. 44, 464–473.
| 1:STN:280:DyaL1M7ptVKmuw%3D%3D&md5=0a5d9669a38f67687984ce6a729d13e4CAS |
| 1:STN:280:DyaL1M7ptVKmuw%3D%3D&md5=0a5d9669a38f67687984ce6a729d13e4CAS | 2818710PubMed | 2818710PubMed |
Richter, K. S., Harris, D. C., Daneshmand, S. T., and Shapiro, B. S. (2001). Quantitative grading of a human blastocyst: optimal inner cell mass size and shape. Fertil. Steril. 76, 1157–1167.
| Quantitative grading of a human blastocyst: optimal inner cell mass size and shape.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3Mnps1yjug%3D%3D&md5=e3e85d344870b172135aefa45529b844CAS | 1:STN:280:DC%2BD3Mnps1yjug%3D%3D&md5=e3e85d344870b172135aefa45529b844CAS | 11730744PubMed | 11730744PubMed |
Roux, C., Joanne, C., Agnani, G., Fromm, M., Clavequin, M. C., and Bresson, J. L. (1995). Morphometric parameters of living human in vitro fertilisation embryos; importance of the asynchronous division process. Hum. Reprod. 10, 1201–1207.
| 1:STN:280:DyaK2Mzos1KntA%3D%3D&md5=76c5bc5c5f8737049054b6a84c3f4126CAS |
| 1:STN:280:DyaK2Mzos1KntA%3D%3D&md5=76c5bc5c5f8737049054b6a84c3f4126CAS | 7657766PubMed | 7657766PubMed |
Rubio, C., Rodrigo, L., Mercader, A., Mateu, E., Buendia, P., Pehlivan, T., Viloria, T., De los Santos, M. J., Simon, C., Remohi, J., and Pellicer, A. (2007). Impact of chromosomal abnormalities on preimplantation embryo development. Prenat. Diagn. 27, 748–756.
| Impact of chromosomal abnormalities on preimplantation embryo development.Crossref | GoogleScholarGoogle Scholar | 17546708PubMed | 17546708PubMed |
Scott, L. (2003). Pronuclear scoring as a predictor of embryo development. Reprod. Biomed. Online 6, 201–214.
| Pronuclear scoring as a predictor of embryo development.Crossref | GoogleScholarGoogle Scholar | 12676001PubMed | 12676001PubMed |
Scott, L., Alvero, R., Leondires, M., and Miller, B. (2000). The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Hum. Reprod. 15, 2394–2403.
| The morphology of human pronuclear embryos is positively related to blastocyst development and implantation.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M%2FmtVahsA%3D%3D&md5=11ca3b6566ebcf7edc2b8f3f6186735eCAS | 1:STN:280:DC%2BD3M%2FmtVahsA%3D%3D&md5=11ca3b6566ebcf7edc2b8f3f6186735eCAS | 11056141PubMed | 11056141PubMed |
Scott, L., Finn, A., O’Leary, T., McLellan, S., and Hill, J. (2007). Morphologic parameters of early cleavage-stage embryos that correlate with fetal development and delivery: prospective and applied data for increased pregnancy rates. Hum. Reprod. 22, 230–240.
| Morphologic parameters of early cleavage-stage embryos that correlate with fetal development and delivery: prospective and applied data for increased pregnancy rates.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28jitVCqsA%3D%3D&md5=2bd6e587c1dbe1283fdf697f839c7f05CAS | 1:STN:280:DC%2BD28jitVCqsA%3D%3D&md5=2bd6e587c1dbe1283fdf697f839c7f05CAS | 16982662PubMed | 16982662PubMed |
Scriven, P. N., Handyside, A. H., and Ogilvie, C. M. (1998). Chromosome translocations: segregation modes and strategies for preimplantation genetic diagnosis. Prenat. Diagn. 18, 1437–1449.
| Chromosome translocations: segregation modes and strategies for preimplantation genetic diagnosis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXht1Cms74%3D&md5=e28807fec024b3c05986b097b4ee0440CAS | 1:CAS:528:DyaK1MXht1Cms74%3D&md5=e28807fec024b3c05986b097b4ee0440CAS | 9949444PubMed | 9949444PubMed |
Senn, A., Urner, F., Chanson, A., Primi, M. P., Wirthner, D., and Germond, M. (2006). Morphological scoring of human pronuclear zygotes for prediction of pregnancy outcome. Hum. Reprod. 21, 234–239.
| Morphological scoring of human pronuclear zygotes for prediction of pregnancy outcome.Crossref | GoogleScholarGoogle Scholar | 16126750PubMed | 16126750PubMed |
Sjöblom, P., Menezes, J., Cummins, L., Mathiyalagan, B., and Costello, M. F. (2006). Prediction of embryo developmental potential and pregnancy based on early-stage morphological characteristics. Fertil. Steril. 86, 848–861.
| Prediction of embryo developmental potential and pregnancy based on early-stage morphological characteristics.Crossref | GoogleScholarGoogle Scholar | 17027355PubMed | 17027355PubMed |
Staessen, C., and Van Steirteghem, A. (1998). The genetic constitution of multinuclear blastomeres and their derivative daughter blastomeres. Hum. Reprod. 13, 1625–1631.
| The genetic constitution of multinuclear blastomeres and their derivative daughter blastomeres.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1czltFKruw%3D%3D&md5=71e70ce60e61ae87eff3ec774dc3e04fCAS | 1:STN:280:DyaK1czltFKruw%3D%3D&md5=71e70ce60e61ae87eff3ec774dc3e04fCAS | 9688403PubMed | 9688403PubMed |
Stern, C., Pertile, M., Norris, H., Hale, L., and Baker, H. W. (1999). Chromosome translocations in couples with in vitro fertilisation implantation failure. Hum. Reprod. 14, 2097–2101.
| Chromosome translocations in couples with in vitro fertilisation implantation failure.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1MzmslGnsg%3D%3D&md5=969b6cd860c036f90ebb6ba9971654b1CAS | 1:STN:280:DyaK1MzmslGnsg%3D%3D&md5=969b6cd860c036f90ebb6ba9971654b1CAS | 10438432PubMed | 10438432PubMed |
Wei, Y., Multi, S., Yang, C. R., Ma, J., Zhang, Q. H., Wang, Z. B., Li, M., Wei, L., Ge, Z. J., Zhang, C. H., Ouyang, Y. C., Hou, Y., Schatten, H., and Sun, Q. Y. (2011). Spindle assembly checkpoint regulates mitotic cell cycle progression during preimplantation embryo development. PLoS One 6, e21557.
| Spindle assembly checkpoint regulates mitotic cell cycle progression during preimplantation embryo development.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXot1yrsLk%3D&md5=aa78936528b44213c6713c41af67ac77CAS | 1:CAS:528:DC%2BC3MXot1yrsLk%3D&md5=aa78936528b44213c6713c41af67ac77CAS | 21720555PubMed | 21720555PubMed |
Wong, C. C., Loewke, K. E., Bossert, N. L., Behr, B., De Jonge, C. J., Baer, T. M., and Reijo Pera, R. A. (2010). Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat. Biotechnol. 28, 1115–1121.
| Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1elurfF&md5=f37312bca6b7ac68bbbb5624ad070d4dCAS | 1:CAS:528:DC%2BC3cXht1elurfF&md5=f37312bca6b7ac68bbbb5624ad070d4dCAS | 20890283PubMed | 20890283PubMed |
Wright, G., Wiker, S., Elsner, C., Kort, H., Massey, J., Mitchell, D., Toledo, A., and Cohen, J. (1990). Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation. Hum. Reprod. 5, 109–115.
| 1:STN:280:DyaK3c3hs12rtg%3D%3D&md5=1a5a999c85293b9306c8b78fc35b8ce2CAS |
| 1:STN:280:DyaK3c3hs12rtg%3D%3D&md5=1a5a999c85293b9306c8b78fc35b8ce2CAS | 2324240PubMed | 2324240PubMed |
Zollner, U., Zollner, K. P., Hartl, G., Dietl, J., and Steck, T. (2002). The use of a detailed zygote score after IVF/ICSI to obtain good-quality blastocysts: the German experience. Hum. Reprod. 17, 1327–1333.
| The use of a detailed zygote score after IVF/ICSI to obtain good-quality blastocysts: the German experience.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD383ktlajsg%3D%3D&md5=a3aaf4b042c19846d6eea9b4a6a88486CAS | 1:STN:280:DC%2BD383ktlajsg%3D%3D&md5=a3aaf4b042c19846d6eea9b4a6a88486CAS | 11980760PubMed | 11980760PubMed |
