The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality
Urszula Marzec-Wróblewska A B , Piotr Kamiński A C L , Paweł Łakota D , Marek Szymański E F , Karolina Wasilow F G , Grzegorz Ludwikowski H , Magdalena Kuligowska-Prusińska I , Grażyna Odrowąż-Sypniewska I , Tomasz Stuczyński A J and Alina Woźniak K
+ Author Affiliations
- Author Affiliations
A Department of Ecology and Environmental Protection, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.
B Department of Biopharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Dr A. Jurasz St. 2, PL 85-089 Bydgoszcz, Poland.
C Department of Biotechnology, Faculty of Biological Sciences, University of Zielona Góra, Prof. Szafran St. 1, PL 65-516 Zielona Góra, Poland.
D Department of Animal Biotechnology, Faculty of Animal Biology, University of Technology and Life Sciences, Mazowiecka St. 28, PL 85-084 Bydgoszcz, Poland.
E Department of Obstetrics, Female Pathology and Oncological Gynecology, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Ujejski St. 75, PL 85-168 Bydgoszcz, Poland.
F NZOZ Medical Center Co. – Clinic of Infertility Treatment “Genesis”, Waleniowa St. 24, PL 85-435 Bydgoszcz, Poland.
G Family Medicine Clinic, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Ujejski St. 75, PL 85-168 Bydgoszcz, Poland.
H Department of Clinical Andrology, University Hospital No. 2, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Szpitalna St. 19, PL 85-092 Bydgoszcz, Poland.
I Department of Laboratory Diagnostics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowska-Curie St. 9, PL 85-094 Bydgoszcz, Poland.
J Department of Soil Structure, Institute of Agriculture and Soil Cultivation, Czartoryskich St. 8, PL 24-100 Puławy, Poland.
K Department of Medical Biology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Karłowicz St. 24, PL 85-092 Bydgoszcz, Poland.
L Corresponding author. Email: piotr.kaminski@cm.umk.pl
Reproduction, Fertility and Development 28(10) 1518-1525 https://doi.org/10.1071/RD15041
Submitted: 3 June 2014 Accepted: 8 February 2015 Published: 19 March 2015
Abstract
We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.
Additional keywords: ejaculate parameters, glutathione peroxidase, male infertility, semen, seminal plasma.
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