Activation of P2X7 receptors decreases the proliferation of murine luteal cells
Jing Wang A , Shuangmei Liu B , Yijun Nie C , Bing Wu B , Qin Wu B , Miaomiao Song B , Min Tang D , Li Xiao D , Ping Xu E , Ximin Tan E , Luyin Zhang E , Gang Li D , Shangdong Liang B and Chunping Zhang D F
+ Author Affiliations
- Author Affiliations
A Department of Microbiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People’s Republic of China.
B Department of Physiology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People’s Republic of China.
C The First Affiliated Hospital, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People’s Republic of China.
D Department of Cell Biology, School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People’s Republic of China.
E School of Medicine, Nanchang University, #461 Bayi Avenue, Nanchang, Jiangxi 330006, People’s Republic of China.
F Corresponding author. Email: zhangcp81@gmail.com
Reproduction, Fertility and Development 27(8) 1262-1271 https://doi.org/10.1071/RD14381
Submitted: 9 October 2014 Accepted: 9 February 2015 Published: 18 March 2015
Abstract
Extracellular ATP regulates cellular function in an autocrine or paracrine manner through activating purinergic signalling. Studies have shown that purinergic receptors were expressed in mammalian ovaries and they have been proposed as an intra-ovarian regulatory mechanism. P2X7 was expressed in porcine ovarian theca cells and murine and human ovarian surface epithelium and is involved in ATP-induced apoptotic cell death. However, the role of P2X7 in corpus luteum is still unclear. The aim of this study was to investigate the role of ATP signalling in murine luteal cells and the possible mechanism(s) involved. We found that P2X7 was highly expressed in murine small luteal cells. The agonists of P2X7, ATP and BzATP, inhibited the proliferation of luteal cells. P2X7 antagonist BBG reversed the inhibition induced by ATP and BzATP. Further studies showed that ATP and BzATP inhibited the expression of cell cycle regulators cyclinD2 and cyclinE2. ATP and BzATP also inhibited the p38–mitogen-activated protein kinase (MAPK) signalling pathway. These results reveal that P2X7 receptor activation is involved in corpus luteum formation and function.
Additional keywords: ATP, corpus luteum, cyclinD2, p38MAPK, purinergic signaling.
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