Transcriptome of early embryonic invasion at implantation sites in a murine model
J. M. Moreno-Moya A , N. A. Franchi B E , S. Martínez-Escribano A , J. A. Martínez-Conejero A , S. Bocca B , S. Oehninger B and J. A. Horcajadas C D F
+ Author Affiliations
- Author Affiliations
A Fundación IVI (FIVI)–Instituto Universitario IVI (IUIVI), University of Valencia, Parc Científic Universitat de València, Catedrático Agustín Escardino, 9, 46980 – Paterna, Valencia, Spain.
B The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA 23507, USA.
C Araid at I+CS and Unviersidad Pablo de Olavide, Sevilla, Hospital Miguel Servet, Zaragoza, Spain.
D Universidad Pablo de Olavide, Sevilla, Spain.
E Present address: Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT), CONICET and Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.
F Corresponding author. Email: jose.horcajadas@gmail.com
Reproduction, Fertility and Development 28(10) 1487-1498 https://doi.org/10.1071/RD14166
Submitted: 20 May 2014 Accepted: 23 January 2015 Published: 5 May 2015
Abstract
Successful implantation relies on the interaction between a competent embryo and a receptive endometrium. The aim of the present study was to investigate genes differentially expressed in early invasive embryonic tissue versus decidual tissue in mice. Samples were obtained from the ectoplacental cone, the immediately surrounding deciduas and from deciduas from interimplantation sites. Microarray analysis showed that 817 genes were differentially expressed between extra-embryonic tissue and the surrounding decidua and that 360 genes were differentially expressed between the different deciduas, with a high representation of developmental processes. Genes differentially expressed in the maternal compartment included chemokines, lipoproteins, growth factors and transcription factors, whereas the embryonic invasive tissue expressed genes commonly observed in invasive tumour-like processes. These results provide information about genes involved in early embryonic invasion and the control exerted by the surrounding decidua. This information may be useful to find targets involved in pathologies associated with implantation failure and early pregnancy loss.
Additional keywords: ectoplacental cone, decidual tissue, microarray, mouse implantation, RNA expression patterns, trophoblast invasion.
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