Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems
Maricy Apparicio A C , Giuliano Q. Mostachio A , Tathiana F. Motheo A , Aracelle E. Alves A , Luciana Padilha A , Eliandra A. Pires-Butler A , Paula A. P. Savi A , Ricardo A. R. Uscategui A , Gaia C. Luvoni B and Wilter R. R. Vicente A
+ Author Affiliations
- Author Affiliations
A UNESP – Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
B Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
C Corresponding author. Email: maricyap@fcav.unesp.br
Reproduction, Fertility and Development 27(7) 1082-1087 https://doi.org/10.1071/RD14022
Submitted: 21 January 2014 Accepted: 13 March 2014 Published: 28 April 2014
Abstract
The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72 h in base medium (BM) with 10 IU mL–1 human chorionic gonadotrophin (hCG), 1 μg mL–1 progesterone (P4) and 1 μg mL–1 oestradiol (E2); in bi-phasic System B oocytes were matured for 48 h in BM with hCG and for 24 h in BM with P4; in bi-phasic System C oocytes were matured for 48 h in BM with hCG, P4 and E2, and for 24 h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P < 0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P < 0.001). The stage of the oestrous cycle did not influence maturation rates.
Additonal keywords: hormones, in vitro maturation.
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