Interaction between differential gene expression profile and phenotype in bovine blastocysts originating from oocytes exposed to elevated non-esterified fatty acid concentrations
V. Van Hoeck A , D. Rizos B , A. Gutierrez-Adan B , I. Pintelon A , E. Jorssen A , I. Dufort C , M. A. Sirard C , A. Verlaet D , N. Hermans D , P. E. J. Bols A and J. L. M. R. Leroy A E
+ Author Affiliations
- Author Affiliations
A Department of Veterinary Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
B Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra. de la Coruna Km 5.9, 28040 Madrid, Spain.
C Département des Sciences Animales Pavillon des services, INAF, Université Laval, G1V 0A6 Québec, Canada.
D Departement Pharmaceutical Sciences, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
E Corresponding author. Email: jo.leroy@uantwerpen.be
Reproduction, Fertility and Development 27(2) 372-384 https://doi.org/10.1071/RD13263
Submitted: 13 August 2013 Accepted: 28 October 2013 Published: 23 December 2013
Abstract
Maternal metabolic disorders linked to lipolysis are major risk factors for reproductive failure. A notable feature of such disorders is increased non-esterified fatty acid (NEFA) concentrations in the blood, which are reflected in the ovarian follicular fluid. Elevated NEFA concentrations impact on the maturing oocyte and even alter subsequent embryo physiology. The aetiological mechanisms have not been fully elucidated. Therefore, in the present study, bovine in vitro maturing cumulus–oocyte complexes were exposed (24 h) to three different maturation treatments containing: (1) physiological (72 µM) NEFA concentrations (= control); (2) elevated (75 µM) stearic acid (SA) concentrations (= HIGH SA); and (3) elevated (425 µM) NEFA concentrations (= HIGH COMBI). Zygotes were fertilised and cultured following standard procedures. Transcriptomic analyses in resulting Day 7.5 blastocysts revealed that the major pathways affected are related to lipid and carbohydrate metabolism in HIGH COMBI embryos and to lipid metabolism and cell death in HIGH SA embryos. Furthermore, lower glutathione content and a reduced number of lipid droplets per cell were observed in HIGH SA-exposed oocytes and resulting morulae, respectively, compared with their HIGH COMBI-exposed counterparts. Vitrified embryos originating from HIGH SA-exposed oocytes tended to exhibit lower survival rates compared with controls. These data suggest possible mechanisms explaining why females across species suffering lipolytic disorders experience difficulties in conceiving.
Additional keywords: embryo quality, fertility, lipolysis, maternal metabolism.
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