The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen
Haixia Jin A , Yimin Shu B , Shanjun Dai A , Zhaofeng Peng A , Senlin Shi A and Yingpu Sun A C
+ Author Affiliations
- Author Affiliations
A Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.
B Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA.
C Corresponding author. Email: syp2008@vip.sina.com
Reproduction, Fertility and Development 26(2) 346-350 https://doi.org/10.1071/RD12369
Submitted: 14 November 2012 Accepted: 21 January 2013 Published: 28 March 2013
Abstract
In this study we evaluated the value of short-time insemination and early rescue intra-cytoplasmic sperm injection (ICSI) in preventing the occurrence of complete fertilisation failure for mild or moderate male infertility patients. A total of 866 couples with borderline semen who underwent in vitro fertilisation treatment in 2010 were included. Regular insemination was performed between January and June of 2010 and short-term insemination was performed from July through December 2010, where, as early as 4 h after insemination, oocytes were denuded from cumulus cells and extrusion of the second polar body was evaluated. Of the 4153 mature oocytes with a detectable second polar body 4 h after insemination, 3874 (93.3%) showed signs of fertilisation on Day 1. Where no second polar body was present in any of the retrieved oocytes for a given patient, rescue ICSI was performed immediately. Similar rates of normal fertilisation and percentage of good-quality embryos were obtained between early rescue ICSI and regular ICSI. Clinical pregnancy occurred in 16 of 43 patients (37.2%) receiving early rescue ICSI. Our results showed early rescue ICSI in combination with evaluation of the second polar body 4 h following insemination is an effective method to prevent complete fertilisation failure for patients with mild or moderate male infertility.
Additional keywords: borderline semen, clinical outcome, early rescue ICSI, failed fertilization, second polar body.
References
Chen, C., and Kattera, S. (2003). Rescue ICSI of oocytes that failed to extrude the second polar body 6 h post-insemination in conventional IVF. Hum. Reprod. 18, 2118–2121.| Rescue ICSI of oocytes that failed to extrude the second polar body 6 h post-insemination in conventional IVF.Crossref | GoogleScholarGoogle Scholar | 14507831PubMed |
Dai, S. J., Qiao, Y. H., Jin, H. X., Xin, Z. M., Su, Y. C., Sun, Y. P., and Chian, R. C. (2012). Effect of co-incubation time of sperm–oocytes on fertilization, embryonic development and subsequent pregnancy outcome. Syst. Biol. Reprod. Med. 58, 348–353.
| Effect of co-incubation time of sperm–oocytes on fertilization, embryonic development and subsequent pregnancy outcome.Crossref | GoogleScholarGoogle Scholar | 22856526PubMed |
Dirnfeld, M., Bider, D., Koifman, M., Calderon, I., and Abramovici, H. (1999). Shortened exposure of oocytes to spermatozoa improves in vitro fertilization outcome: a prospective, randomized, controlled study. Hum. Reprod. 14, 2562–2564.
| Shortened exposure of oocytes to spermatozoa improves in vitro fertilization outcome: a prospective, randomized, controlled study.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1MvlvFemsg%3D%3D&md5=dab7314a1b8785eab4069504814870efCAS | 10527987PubMed |
Downs, S. M., Gilles, R., Vanderhoef, C., Humpherson, P. G., and Leese, H. J. (2006). Differential response of cumulus cell-enclosed and denuded mouse oocytes in a meiotic induction model system. Mol. Reprod. Dev. 73, 379–389.
| Differential response of cumulus cell-enclosed and denuded mouse oocytes in a meiotic induction model system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhsVKhtLo%3D&md5=4f3bbdb0c569f92abe5a4b71723478dbCAS | 16362973PubMed |
Gardner, A. J., and Evans, J. P. (2006). Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm. Reprod. Fertil. Dev. 18, 53–61.
| Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlanu7vI&md5=5bc53f18a8529f4be5467d6bd7b85bafCAS | 16478602PubMed |
Hershlag, A., Paine, T., Kvapil, G., and Feng, H. (2002). Napolitano B. In vitro fertilization-intracytoplasmic sperm injection split: an insemination method to prevent fertilization failure. Fertil. Steril. 77, 229–232.
| Napolitano B. In vitro fertilization-intracytoplasmic sperm injection split: an insemination method to prevent fertilization failure.Crossref | GoogleScholarGoogle Scholar | 11821076PubMed |
Kattera, S., and Chen, C. (2003). Short co-incubation of gametes in in vitro fertilization improves implantation and pregnancy rates: a prospective, randomized, controlled study. Fertil. Steril. 80, 1017–1021.
| Short co-incubation of gametes in in vitro fertilization improves implantation and pregnancy rates: a prospective, randomized, controlled study.Crossref | GoogleScholarGoogle Scholar | 14556826PubMed |
Kuczyński, W., Dhont, M., Grygoruk, C., Pietrewicz, P., Redzko, S., and Szamatowicz, M. (2002). Rescue ICSI of unfertilized oocytes after IVF. Hum. Reprod. 17, 2423–2427.
| Rescue ICSI of unfertilized oocytes after IVF.Crossref | GoogleScholarGoogle Scholar | 12202435PubMed |
Morton, P. C., Yoder, C. S., Tucker, M. J., Wright, G., Brockman, W. D., and Kort, H. I. (1997). Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure. Fertil. Steril. 68, 488–491.
| Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2svmtVGgug%3D%3D&md5=12be8c82ec4ebb9ee29e6f9c4c4ffdd7CAS | 9314920PubMed |
Nagy, Z. P., Staessen, C., Liu, J., Joris, H., Devroey, P., and Van Steirteghem, A. C. (1995). Prospective, auto-controlled study on reinsemination of failed-fertilized oocytes by intracytoplasmic sperm injection. Fertil. Steril. 64, 1130–1135.
| 1:STN:280:DyaK28%2FmvFGmuw%3D%3D&md5=3698abc11aa87e4f0ed5a8cded253dd7CAS | 7589665PubMed |
Nagy, Z. P., Rienzi, L. F., Ubaldi, F. M., Greco, E., Massey, J. B., Kort, H. I., Nagy, Z. P., Rienzi, L. F., Ubaldi, F. M., Greco, E., Massey, J. B., and Kort, H. I. (2006). Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection. Fertil. Steril. 85, 901–906.
| Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 16580372PubMed |
Park, K. S., Song, H. B., and Chun, S. S. (2000). Late fertilization of unfertilized human oocytes in in vitro fertilization and intracytoplasmic sperm injection cycles: conventional insemination versus ICSI. J. Assist. Reprod. Genet. 17, 419–424.
| Late fertilization of unfertilized human oocytes in in vitro fertilization and intracytoplasmic sperm injection cycles: conventional insemination versus ICSI.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3crhtVelsA%3D%3D&md5=d07913bfa66f689b6b8289cbb4dc0251CAS | 11062851PubMed |
Plachot, M., Belaisch-Allart, J., Mayenga, J. M., Chouraqui, A., Tesquier, L., and Serkine, A. M. (2002). Outcome of conventional IVF and ICSI on sibling oocytes in mild male factor infertility. Hum. Reprod. 17, 362–369.
| Outcome of conventional IVF and ICSI on sibling oocytes in mild male factor infertility.Crossref | GoogleScholarGoogle Scholar | 11821279PubMed |
Scott, L. (2003). Pronuclear scoring as a predictor of embryo development. Reprod. Biomed. Online 6, 201–214.
| Pronuclear scoring as a predictor of embryo development.Crossref | GoogleScholarGoogle Scholar | 12676001PubMed |
Suppinyopong, S., Choavaratana, R., and Karavakul, C. (2000). Timing of second polar body extrusion and pronuclear formation after intracytoplasmic sperm injection (ICSI). J. Med. Assoc. Thai. 83, 500–503.
| 1:STN:280:DC%2BD3czit1agtg%3D%3D&md5=4e1fa38ed6bc2cd971f43f89f54ec54eCAS | 10863895PubMed |
Van Blerkom, J., Davis, P. W., and Merriam, J. (1994). The developmental ability of human oocytes penetrated at the germinal vesicle stage after insemination in vitro. Hum. Reprod. 9, 697–708.
| 1:STN:280:DyaK2czitFGgtQ%3D%3D&md5=5d94c788ddec9dc991725c6b0b498e76CAS | 8046026PubMed |
Van den Bergh, M., Bertrand, E., and Englert, Y. (1995). Second polar body extrusion is highly predictive for oocyte fertilization as soon as 3 hr after intracytoplasmic sperm injection (ICSI). J. Assist. Reprod. Genet. 12, 258–262.
| Second polar body extrusion is highly predictive for oocyte fertilization as soon as 3 hr after intracytoplasmic sperm injection (ICSI).Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK28%2FlvFKmtQ%3D%3D&md5=ca8851cbe13d576bb520baee2762470bCAS | 7580022PubMed |
Wang, W. H., Day, B. N., and Wu, G. M. (2003). How does polyspermy happen in mammalian oocytes? Microsc. Res. Tech. 61, 335–341.
| How does polyspermy happen in mammalian oocytes?Crossref | GoogleScholarGoogle Scholar | 12811738PubMed |
World Health Organization (WHO) (1999). ‘World Health Organization Laboratory Manual for Human Semen and Sperm Cervical–Mucus Interaction’. 4th edn. (Cambridge University Press: Cambridge, UK.)
Yuzpe, A. A., Liu, Z., and Fluker, M. R. (2000). Rescue intracytoplasmic sperm injection (ICSI) – salvaging in vitro fertilization (IVF) cycles after total or near total fertilization failure. Fertil. Steril. 73, 1115–1119.
| Rescue intracytoplasmic sperm injection (ICSI) – salvaging in vitro fertilization (IVF) cycles after total or near total fertilization failure.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3czhsF2hsw%3D%3D&md5=3bf6c1bf17c375b2d155d2ae579b3560CAS | 10856467PubMed |
Zhu, L., Xi, Q., Nie, R., Chen, W., Zhang, H., and Li, Y. (2011). Rescue intracytoplasmic sperm injection: a prospective randomized study. J. Reprod. Med. 56, 410–414.
| 22010525PubMed |
