Triphenyltin chloride induces spindle microtubule depolymerisation and inhibits meiotic maturation in mouse oocytes
Yu-Ting Shen A F , Yue-Qiang Song B F , Xiao-Qin He A , Fei Zhang A , Xin Huang D , Yu Liu D , Lu Ding B , Lin Xu D , Mao-Bi Zhu E , Wen-Feng Hu A , Zhong-Quan Qi D , Hai-Long Wang C D G and Xiang-Jun Yang A G
+ Author Affiliations
- Author Affiliations
A Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen, Fujian Province, 361004, China.
B Center of Reproductive Medicine, Women and Children Health Care Hospital of Xiamen, Xiamen, Fujian Province, 361003, China.
C Basic Medical Department of Medical College, Xiamen University, Xiamen, Fujian Province, 361102, China.
D Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian Province, 361102China.
E Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, 361003, China.
F These authors contributed equally to this work.
G Corresponding authors. Emails: xingyun207@126.com; hailongwang@xmu.edu.cn
Reproduction, Fertility and Development 26(8) 1084-1093 https://doi.org/10.1071/RD12332
Submitted: 12 October 2012 Accepted: 1 August 2013 Published: 28 August 2013
Abstract
Meiosis produces haploid gametes for sexual reproduction. Triphenyltin chloride (TPTCL) is a highly bioaccumulated and toxic environmental oestrogen; however, its effect on oocyte meiosis remains unknown. We examined the effect of TPTCL on mouse oocyte meiotic maturation in vitro and in vivo. In vitro, TPTCL inhibited germinal vesicle breakdown (GVBD) and first polar body extrusion (PBE) in a dose-dependent manner. The spindle microtubules completely disassembled and the chromosomes condensed after oocytes were exposed to 5 or 10 μg mL–1 TPTCL. γ-Tubulin protein was abnormally localised near chromosomes rather than on the spindle poles. In vivo, mice received TPTCL by oral gavage for 10 days. The general condition of the mice deteriorated and the ovary coefficient was reduced (P < 0.05). The number of secondary and mature ovarian follicles was significantly reduced by 10 mg kg–1 TPTCL (P < 0.05). GVBD decreased in a non-significant, dose-dependent manner (P > 0.05). PBE was inhibited with 10 mg kg–1 TPTCL (P < 0.05). The spindles of in vitro and in vivo metaphase II oocytes were disassembled with 10 mg kg–1 TPTCL. These results suggest that TPTCL seriously affects meiotic maturation by disturbing cell-cycle progression, disturbing the microtubule cytoskeleton and inhibiting follicle development in mouse oocytes.
Additional keywords: chromosome alignment, immunofluorescent staining, oocyte meiosis, spindle assembly.
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