Differential expression of the P2X7 receptor in ovarian surface epithelium during the oestrous cycle in the mouse
F. G. Vázquez-Cuevas A , A. Cruz-Rico A , E. Garay A , A. García-Carrancá B , D. Pérez-Montiel C , B. Juárez A and R. O. Arellano A D
+ Author Affiliations
- Author Affiliations
A Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Juriquilla Querétaro, CP, 76230, Querétaro México.
B Laboratorio de Virus y Cáncer, Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas–Universidad Nacional Autónoma de México and División de Investigación Básica, Instituto Nacional de Cancerología, Secretaría de Salud, México. Av. San Fernando #22, Colonia Sección XVI, Tlalpan, CP, 14080, México DF.
C Departamento de Patología, Instituto Nacional de Cancerología, Secretaría de Salud, México. Av. San Fernando #22, Colonia Sección XVI, Tlalpan, CP, 14080, México DF.
D Corresponding author. Email: arellano.ostoa@comunidad.unam.mx
Reproduction, Fertility and Development 25(7) 971-984 https://doi.org/10.1071/RD12196
Submitted: 21 June 2012 Accepted: 4 September 2012 Published: 11 October 2012
Abstract
Purinergic signalling has been proposed as an intraovarian regulatory mechanism. Of the receptors responsible for purinergic transmission, the P2X7 receptor is an ATP-gated cationic channel that displays a broad spectrum of cellular functions ranging from apoptosis to cell proliferation and tumourigenesis. In the present study, we investigated the functional expression of P2X7 receptors in ovarian surface epithelium (OSE). P2X7 protein was detected in the OSE layer of the mouse, both in situ and in primary cultures. In cultures, 2′(3′)-O-(4-Benzoylbenzoyl)adenosine-5′-triphosphate (BzATP) activation of P2X7 receptors increased [Ca2+]i and induced apoptosis. The functionality of the P2X7 receptor was investigated in situ by intrabursal injection of BzATP on each day of the oestrous cycle and evaluation of apoptosis 24 h using the terminal deoxyribonucleotidyl transferase-mediated dUTP–fluorescein nick end-labelling (TUNEL) assay. Maximum effects of BzATP were observed during pro-oestrus, with the effects being blocked by A438079, a specific P2X7 receptor antagonist. Immunofluorescence staining for P2X7 protein revealed more robust expression during pro-oestrus and in OSE regions behind the antral follicles, strongly supporting the notion that the differences in apoptosis can be explained by increased receptor expression, which is regulated during the oestrous cycle. Finally, P2X7 receptor expression was detected in the OSE layer of human ovaries, with receptor expression maintained in human ovaries diagnosed with cancer, as well as in the human ovarian carcinoma SKOV3 cell line.
Additional keywords : ATP, purinergic signalling.
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