Microtubule organisation, pronuclear formation and embryonic development of mouse oocytes after intracytoplasmic sperm injection or parthenogenetic activation and then slow-freezing with 1,2-propanediol
Dun-Gao Li A C D , Yan Zhu B D , Feng-Ying Xing A , Shan-Gang Li A , Xue-Jin Chen A E and Man-Xi Jiang A E
+ Author Affiliations
- Author Affiliations
A Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
B Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Committee, Shanghai Institute of Planned Parenthood Research, 2140 Xie Tu Road, Shanghai 200032, China.
C College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China.
D These authors contributed equally to this work.
E Corresponding author. Emails: manxijiang2002@yahoo.com.cn; chenxuej@yahoo.com.cn
Reproduction, Fertility and Development 25(4) 609-616 https://doi.org/10.1071/RD12124
Submitted: 22 March 2012 Accepted: 7 May 2012 Published: 28 May 2012
Abstract
The goal of this study was to investigate the effect of cryopreservation on oocytes at different times after intracytoplasmic sperm injection (ICSI) and parthenogenetic activation. The study was performed in mouse oocytes fertilised by ICSI, or in artificially-activated oocytes, which were cryopreserved immediately, one hour or five hours later through slow-freezing. After thawing, the rates of survival, fertilisation–activation, embryonic development of oocytes–zygotes and changes in the cytoskeleton and ploidy were observed. Our results reveal a significant difference in survival rates of 0-, 1- and 5-h cryopreserved oocytes following ICSI and artificial activation. Moreover, significant differences in two pronuclei (PN) development existed between the 0-, 1- and 5-h groups of oocytes frozen after ICSI, while the rates of two-PN development of activated oocytes were different between the 1-h and 5-h groups. Despite these initial differences, there was no difference in the rate of blastocyst formation from two-PN zygotes following ICSI or artificial activation. However, compared with ICSI or artificially-activated oocytes cryopreserved at 5 h, many oocytes from the 0- and 1-h cryopreservation groups developed to zygotes with abnormal ploidy; this suggests that too little time before cryopreservation can result in some activated oocytes forming abnormal ploidy. However, our results also demonstrate that spermatozoa can maintain normal fertilisation capacity in frozen ICSI oocytes and the procedure of freeze–thawing did not affect the later development of zygotes.
Additional keywords: ICSI, ploidy, spindle.
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