Abnormal expression of uncoupling protein-2 correlates with CYP11A1 expression in polycystic ovary syndrome
Yun Liu A , Hong Jiang B , Ling-Yun He A , Wu-Jian Huang A , Xiao-Yu He A and Fu-Qi Xing C D
+ Author Affiliations
- Author Affiliations
A Center for Reproductive Medicine, Fuzhou General Hospital, No. 156 Xi’erhuan North Road, Fuzhou 350025, Fujian Province, China.
B Department of Vasculocardiology, Fuzhou General Hospital, No. 156 Xi’erhuan North Road, Fuzhou 350025, Fujian Province, China.
C Center for Reproductive Medicine, Nanfang Hospital, Southern Medical University, Tonghe Road 1838, Guangzhou 510515, Guangdong Province, China.
D Corresponding author. Email: liuyun95@yahoo.com.cn
Reproduction, Fertility and Development 23(4) 520-526 https://doi.org/10.1071/RD10266
Submitted: 18 October 2010 Accepted: 2 November 2010 Published: 11 April 2011
Abstract
Polycystic ovary syndrome (PCOS) may result from hypersensitivity to insulin, which is negatively regulated by uncoupling protein (UCP)-2. Because cholesterol side-chain cleavage enzyme (CYP11A1) is closely linked to PCOS, the expression of UCP-2 and CYP11A1 in ovarian tissues from PCOS patients was examined in the present study. Twelve PCOS patients with hyperandrogenaemia who underwent laparoscopic ovarian wedge resection and 12 age-matched control patients who underwent contralateral ovarian biopsy were enrolled in the study. UCP-2 expression in early stage (primordial, primary and secondary) and late stage (sinus and mature) follicles was examined using immunohistochemistry, whereas UCP-2 and CYP11A1 mRNA and protein levels in ovarian tissue were determined using quantitative reverse transcription–polymerase chain reaction and western blot analyses, respectively. UCP-2 expression increased significantly with follicular development in both control and PCOS tissue, with expression in early stage follicles from PCOS patients significantly greater than that in controls. In addition, both UCP-2 and CYP11A1mRNA and protein levels, mean fasting blood glucose concentrations and fasting serum insulin levels were significantly higher in PCOS patients compared with the control group. Finally, a significant correlation between UCP-2 and CYP11A1 expression was found in PCOS but not control patients. In conclusion, in PCOS patients, there was a correlation between UCP-2 and CYP11A1 expression, which was significantly higher than in the control group. These changes in UCP-2 and CYP11A1 expression may mediate follicle development in PCOS.
Additional keywords: cholesterol side-chain cleavage enzyme, hyperandrogenism, insulin resistance.
References
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