Evaluation of the developmental potential of metaphase I oocytes from stimulated intracytoplasmic sperm injection cycles
Mei Li A , Yuan Li A , Shui-Ying Ma A , Huai-Liang Feng B , Hui-Jun Yang A , Ke-Liang Wu A , Wan-Xia Zhong A , Li Che C and Zi-Jiang Chen A D
+ Author Affiliations
- Author Affiliations
A Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People’s Republic of China.
B Department of Obstetrics and Gynaecology, New York University School of Medicine, New York, NY 11030, USA.
C Case Western Reserve University, Cleveland, OH 44106, USA.
D Corresponding author. Email: zjchen59@yahoo.com
Reproduction, Fertility and Development 23(3) 433-437 https://doi.org/10.1071/RD10228
Submitted: 11 September 2010 Accepted: 13 October 2010 Published: 3 February 2011
Abstract
The objective of the present study was to evaluate the developmental potential and clinical application value of metaphase I (MI) oocytes obtained from stimulated intracytoplasmic sperm injection (ICSI) cycles. ICSI was performed on MI oocytes immediately after denudation (Group A), or on in vitro-matured (IVM) oocytes following culture; oocytes in culture were further divided into two groups, being cultured for either 3–5 h (Group B) or 24–28 h (Group C). Metaphase II oocytes from the same cycle(s) isolated for ICSI served as the control group (Group D). The rates of normal fertilisation, cleavage and high-quality embryos were compared among the four groups. High-quality embryos were transferred whenever possible, and pregnancy rates were evaluated. Results showed that normal fertilisation rates for Groups B, C and D were significantly higher than that of Group A (68.6%, 57.8%, 74.5% and 30.1%, respectively; P < 0.01). The rate of high-quality embryos in Group B was comparable with Group D; the rate for Group C was significantly lower than that of the other groups (P < 0.05). Two clinical pregnancies were achieved after transfer of embryos from IVM oocytes. In vitro maturation of MI oocytes for a short period of time may increase the number of available embryos; however, overnight in vitro culture of MI oocytes did not improve results.
Additional keywords: embryo, fertilisation, in vitro maturation.
References
Balakier, H., Sojecki, A., Motamedi, G., and Librach, C. (2004). Time-dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest. Hum. Reprod. 19, 982–987.| Time-dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest.Crossref | GoogleScholarGoogle Scholar | 15033953PubMed |
Cha, K. Y., and Chian, R. C. (1998). Maturation in vitro of immature human oocytes for clinical use. Hum. Reprod. Update 4, 103–120.
| Maturation in vitro of immature human oocytes for clinical use.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1czks12jtA%3D%3D&md5=805f5164a17d5449c58f30deb5028a00CAS | 9683349PubMed |
Chen, S. U., Chen, H. F., Lien, Y. R., Ho, H. N., Chang, H. C., and Yang, Y. S. (2000). Schedule to inject in vitro-matured oocytes may increase pregnancy after intracytoplasmic sperm injection. Arch. Androl. 44, 197–205.
| Schedule to inject in vitro-matured oocytes may increase pregnancy after intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3czitVelsQ%3D%3D&md5=63d859ad8f56b6571432aa2f1dd2b3aeCAS | 10864367PubMed |
De Vos, A., Van de Velde, H., Joris, H., and Van Steirteghem, A. (1999). In vitro-matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum. Reprod. 14, 1859–1863.
| In vitro-matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1MzjtVKrug%3D%3D&md5=07714960952d6144d05c43bd7fd2d050CAS | 10402405PubMed |
Dupont, C., Bavister, B. D., Armant, D. R., and Brenner, C. A. (2009). Rhesus macaque embryos derived from MI oocytes maturing after retrieval display high rates of chromosomal anomalies. Hum. Reprod. 24, 929–935.
| Rhesus macaque embryos derived from MI oocytes maturing after retrieval display high rates of chromosomal anomalies.Crossref | GoogleScholarGoogle Scholar | 19106174PubMed |
Edirisinghe, W. R., Junk, S. M., Matson, P. L., and Yovich, J. L. (1997). Birth from cryopreserved embryos following in vitro maturation of oocytes and intracytoplasmic sperm injection. Hum. Reprod. 12, 1056–1058.
| Birth from cryopreserved embryos following in vitro maturation of oocytes and intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2szjsl2qtg%3D%3D&md5=00834b78d0b9f48260a44e60dadc0019CAS | 9194665PubMed |
Edwards, R. G. (1965). Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human varian oocytes. Nature 208, 349–351.
| Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human varian oocytes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaF287lsVagsg%3D%3D&md5=043dec9084a29a41137079d061484945CAS | 4957259PubMed |
Fridén, B., Hreinsson, J., and Hovatta, O. (2005). Birth of a healthy infant after in vitro oocyte maturation and ICSI in a woman with diminished ovarian response: case report. Hum. Reprod. 20, 2556–2558.
| Birth of a healthy infant after in vitro oocyte maturation and ICSI in a woman with diminished ovarian response: case report.Crossref | GoogleScholarGoogle Scholar | 15905285PubMed |
Garcia-Velasco, J. A., Isaza, V., Vidal, C., Landazábal, A., Remohí, J., Simón, C., and Pellicer, A. (2001). Human ovarian steroid secretion in vivo: effects of GnRH agonist versus antagonist (cetrorelix). Hum. Reprod. 16, 2533–2539.
| Human ovarian steroid secretion in vivo: effects of GnRH agonist versus antagonist (cetrorelix).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XkslektA%3D%3D&md5=c59e2b720703e803bb97246bd87b92bbCAS | 11726570PubMed |
Hashimoto, S. (2009). Application of in vitro maturation to assisted reproductive technology. J. Reprod. Dev. 55, 1–10.
| Application of in vitro maturation to assisted reproductive technology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktlGgtrs%3D&md5=849ad7f3d54edb1482b75bffb6411705CAS | 19276618PubMed |
Huang, F. J., Chang, S. Y., Tsai, M. Y., Lin, Y. C., Kung, F. T., Wu, J. F., and Lu, Y. J. (1999). Relationship of the human cumulus–free oocyte maturational profile with in vitro outcome parameters after intracytoplasmic sperm injection. J. Assist. Reprod. Genet. 16, 483–487.
| Relationship of the human cumulus–free oocyte maturational profile with in vitro outcome parameters after intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1MvlvF2ksg%3D%3D&md5=4da60b82bec057ecf6e227c95caf846eCAS | 10530402PubMed |
Johnson, J. E., Higdon, H. L., and Boone, W. R. (2008). Effect of human granulosa cell co-culture using standard culture media on the maturation and fertilization potential of immature human oocytes. Fertil. Steril. 90, 1674–1679.
| Effect of human granulosa cell co-culture using standard culture media on the maturation and fertilization potential of immature human oocytes.Crossref | GoogleScholarGoogle Scholar | 17953965PubMed |
Kim, B. K., Lee, S. C., Kim, K. J., Han, C. H., and Kim, J. H. (2000). In vitro maturation, fertilization and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil. Steril. 74, 1153–1158.
| In vitro maturation, fertilization and development of human germinal vesicle oocytes collected from stimulated cycles.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M%2FotVGlsg%3D%3D&md5=aadd64506c8fe136070e604a21e6958aCAS | 11119743PubMed |
Levran, D., Farhi, J., Nachum, H., Glezerman, M., and Weissman, A. (2002). Maturation arrest of human oocytes as a cause of infertility. Hum. Reprod. 17, 1604–1609.
| Maturation arrest of human oocytes as a cause of infertility.Crossref | GoogleScholarGoogle Scholar | 12042285PubMed |
Li, Y., Feng, H. L., Cao, Y. J., Zheng, G. J., Yang, Y., Mullen, S. F., Critser, J. K., and Chen, Z. J. (2006). Confocalmicroscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro. Fertil. Steril. 85, 827–832.
| Confocalmicroscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro.Crossref | GoogleScholarGoogle Scholar | 16580356PubMed |
Liu, J., Lu, G., Qian, Y., Mao, Y., and Ding, W. (2003). Pregnancies and births achieved from in vitro-matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles. Fertil. Steril. 80, 447–449.
| Pregnancies and births achieved from in vitro-matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles.Crossref | GoogleScholarGoogle Scholar | 12909513PubMed |
Liu, S., Jiang, J. J., Feng, H. L., Ma, S. Y., Li, M., and Li, Y. (2010). Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system. Fertil. Steril. 93, 2202–2209.
| Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtFSltb%2FI&md5=f4bbc523cc3ce63b5b215a805d4187c3CAS | 19249763PubMed |
Nagy, Z. P., Cecile, J., Liu, J., Loccuer, A., Devroey, P., and Van, S. A. (1996). Pregnancy and birth after intracytoplasmic sperm injection of IVM MI germinal vesicle-stage oocytes: case report. Fertil. Steril. 65, 1047–1050.
| 1:STN:280:DyaK287pvVyltQ%3D%3D&md5=e8cc5a5803db6141fcccf2d38bbe8fd1CAS | 8612833PubMed |
Puissant, F., Van Rysselberge, M., Barlow, P., Deweze, J., and Leroy, F. (1987). Embryo scoring as a prognostic tool in IVF treatment. Hum. Reprod. 2, 705–708.
| 1:STN:280:DyaL1c7jvFKmuw%3D%3D&md5=20e7515b103deb47a091564abb2bc55bCAS | 3437050PubMed |
Rienzi, L., Ubaldi, F., Anniballo, R., Cerulo, G., and Greco, E. (1998). Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection. Hum. Reprod. 13, 1014–1019.
| Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1c3otVylsA%3D%3D&md5=8b3d42bdc130ebcfa199e6a208479141CAS | 9619563PubMed |
Shu, Y., Gebhardt, J., Watt, J., Lyon, J., Dasig, D., and Behr, B. (2007). Fertilization, embryo development and clinical outcome of immature oocytes from stimulated intracytoplasmic sperm injection cycles. Fertil. Steril. 87, 1022–1027.
| Fertilization, embryo development and clinical outcome of immature oocytes from stimulated intracytoplasmic sperm injection cycles.Crossref | GoogleScholarGoogle Scholar | 17261289PubMed |
Son, W. Y., Lee, S. Y., and Lim, J. H. (2005). Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum. Reprod. 20, 3204–3207.
| Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1Cns7%2FM&md5=7c578e3882b51c80227d400e8df25962CAS | 16037117PubMed |
Strassburger, D., Friedle, S., Raziel, A., Kasterstein, E., Schachter, M., and Ron-El, R. (2004). The outcome of ICSI of immature MI oocytes and rescued in vitro-matured MII oocytes. Hum. Reprod. 19, 1587–1590.
| The outcome of ICSI of immature MI oocytes and rescued in vitro-matured MII oocytes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2czhvVOktQ%3D%3D&md5=5f9037110945f8ffa0c0f65dbcb0b002CAS | 15131077PubMed |
Strassburger, D., Goldstein, A., Friedler, S., Raziel, A., Kasterstein, E., Mashevich, M., Schachter, M., Ron-El, R., and Reish, O. (2010). The cytogenetic constitution of embryos derived from immature (metaphase I) oocytes obtained after ovarian hyperstimulation. Fertil. Steril. 94, 971–978.
| The cytogenetic constitution of embryos derived from immature (metaphase I) oocytes obtained after ovarian hyperstimulation.Crossref | GoogleScholarGoogle Scholar | 19505687PubMed |
Vajta, G., and Nagy, Z. P. (2006). Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod. Biomed. Online 12, 779–796.
| Are programmable freezers still needed in the embryo laboratory? Review on vitrification.Crossref | GoogleScholarGoogle Scholar | 16792858PubMed |
Vanhoutte, L., De Sutter, P., Van der Elst, J., and Dhont, M. (2005). Clinical benefit of metaphase I oocytes. Reprod. Biol. Endocrinol. 3, 71.
| Clinical benefit of metaphase I oocytes.Crossref | GoogleScholarGoogle Scholar | 16356175PubMed |
