Regulation of aromatase expression by 1α,25(OH)2 vitamin D3 in rat testicular cells
Leila Zanatta A B , Hélène Bouraïma-Lelong A , Christelle Delalande A , Fátima R. M. B. Silva B and Serge Carreau A C
+ Author Affiliations
- Author Affiliations
A Laboratoire Oestrogènes et Reproduction, Université de Caen Basse-Normandie, EA 2608, INRA, USC 2006, IFR ICORE, 14032 Caen, France.
B Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040-970, Florianópolis – Santa Catarina, Brazil.
C Corresponding author. Email: serge.carreau@unicaen.fr
Reproduction, Fertility and Development 23(5) 725-735 https://doi.org/10.1071/RD10163
Submitted: 30 June 2010 Accepted: 27 January 2011 Published: 23 May 2011
Abstract
It is well known that the vitamin D endocrine system is involved in physiological and biochemical events in numerous tissues, especially gut, bone and kidney but also testis. Therefore, in this study the effect and mechanisms of action of 1α,25(OH)2 vitamin D3 (1,25D) on aromatase gene expression in immature rat Sertoli cells were evaluated. Vitamin D receptor transcripts were present in immature Sertoli cells as well as in adult testicular germ cells and somatic cells. The treatment of immature Sertoli cells with 100 nM 1,25D increased the amount of aromatase transcript, mainly in 30-day-old rats. The protein kinase A (PKA) blocker, H89, partially inhibited the 1,25D effect. The stimulation of aromatase gene expression in 30-day-old Sertoli cells by the agonist 1α,25(OH)2 lumisterol3, and the suppression of the 1,25D effect by the antagonists 1β,25(OH)2 vitamin D3 and (23S)-25-dehydro-1α (OH)-vitamin D3-26,23-lactone suggested, besides a genomic effect of 1,25D, the existence of non-genomic activation of the membrane-bound vitamin D receptor involving the PKA pathway.
Additional keywords: immature Sertoli cells, vitamin D3 analogues, vitamin D receptor (VDR).
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