Incidence of chromosomal abnormalities in bovine blastocysts derived from unsorted and sex-sorted spermatozoa
M. Garcia-Herreros A , T. F. Carter B , D. A. F. Villagómez C , A. D. MacAulay B , D. Rath D , W. A. King B and P. Lonergan A EA School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
B Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph,Guelph, ON N1G 2W1, Canada.
C Departamento de Producción Animal, Centro Universitario de Ciencias Biólogicas y Agropecuarias, Universidad de Guadalajara, Jalisco, México.
D Department of Biotechnology, Institute of Animal Breeding (FAL), Mariensee,Hoeltystr. 10, 31535 Neustadt, Germany.
E Corresponding author. Email: pat.lonergan@ucd.ie
Reproduction, Fertility and Development 22(8) 1272-1278 https://doi.org/10.1071/RD10052
Submitted: 12 March 2010 Accepted: 28 June 2010 Published: 1 October 2010
Abstract
The aim of the present study was to examine the incidence of chromosomal abnormalities in bovine blastocysts produced by IVF with unsorted, X-sorted or Y-sorted spermatozoa. In Experiment 1, individual blastocysts were processed to examine the incidence of mixoploidy using fluorescent in situ hybridisation. Overall, 80% (44/55) of blastocysts were mixoploid (10/15, 14/15 and 20/25 for X-sorted, Y-sorted and unsorted spermatozoa, respectively; P > 0.05). However, the prevalence of abnormal XY chromosome complements was relatively low in all groups; on average, only a small fraction of the total nuclei per embryo appeared polyploid (1.64%, 5.62% and 6.0% for X-sorted, Y-sorted and unsorted spermatozoa, respectively). Interestingly, 20% (5/25) of blastocysts derived from unsorted spermatozoa were found to be chimeric (XX/XY). In Experiment 2, chimeric embryos were detected among the blastocysts derived from two of five sires tested. In addition, one chimeric blastocyst was detected among nine in vivo-derived blastocysts obtained following AI. In conclusion, based on the results of the present study, the incidence of chromosomal abnormalities did not different between blastocysts derived from sex-sorted or unsorted spermatozoa. In addition, the occurrence of mixed sex chimeras was not limited to a single sire and was not unique to blastocysts derived from IVF.
Additional keywords: chromosome aberrations, embryos, in vitro fertilisation.
Acknowledgements
P.L. is funded by Science Foundation Ireland (07/SRC/B1156). MGH was funded by a post-doctoral fellowship from the Spanish Government (2008–0198). The authors thank Mary Wade for excellent technical assistance and to the staff of Kepak (Clonee, County Meath, Ireland) and Kildare Chilling (Kildare Town, Country Kildare, Ireland) for allowing access to bovine tissues.
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