Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis
Claudia Fischer A , Marc Drillich B , Simone Odau A , Wolfgang Heuwieser B C , Ralf Einspanier A and Christoph Gabler A D
+ Author Affiliations
- Author Affiliations
A Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
B Clinic for Animal Reproduction, Freie Universität Berlin, Königsweg 63, 14163 Berlin, Germany.
C Present address: Animal Welfare Program, Faculty of Land and Food Systems, The University of British Columbia, 2357 Main Mall, Vancouver V6T 1Z4, Canada.
D Corresponding author. Email: gablerch@zedat.fu-berlin.de
Reproduction, Fertility and Development 22(5) 818-829 https://doi.org/10.1071/RD09120
Submitted: 14 May 2009 Accepted: 3 November 2009 Published: 7 April 2010
Abstract
Endometrial cells take part in embryo–maternal communication, as well as supporting the immune system in defending against invading pathogens. The aim of the present study was to examine the mRNA expression of factors that have been suggested to be involved in both events in the bovine endometrial epithelium, namely bovine granulocyte chemotactic protein 2 (CXCL5), interleukin-1 beta (IL1B), IL6, IL8, tumour necrosis factor (TNF), cyclooxygenase 2 (PTGS2) and haptoglobin (HP). Samples were collected in vivo from cows on Days 21–27 postpartum by the cytobrush method to evaluate the correlation between inflammatory factors and uterine health (cows with signs of clinical or subclinical endometritis and healthy cows). Bovine uteri were collected at the abattoir to investigate oestrous cycle-dependent mRNA expression patterns. Real-time reverse transcription–polymerase chain reaction revealed that the expression of CXCL5, IL1B, IL8 and TNF mRNA was significantly higher in cows with subclinical or clinical endometritis compared with healthy cows. The expression of CXCL5, IL1B and IL8 mRNA was increased around ovulation compared with the luteal phase. There was no indication of either oestrous cycle-dependent expression or a correlation with uterine health for IL6, PTGS2 and HP transcripts. These results suggest that CXCL5, IL1B, IL8 and TNF may represent potential marker genes for the detection of cows with subclinical endometritis and for monitoring new therapeutic approaches.
Additional keywords: cyclooxygenase, granulocyte chemotactic protein 2, interleukin, oestrous cycle, polymorphonuclear neutrophil, tumour necrosis factor.
Acknowledgements
The authors thank Fleischzentrum Lausitz GmbH (Kasel-Golzig, Germany) for the opportunity to harvest tissue samples and gratefully acknowledge the cooperation of staff at the dairy farm. The authors are also grateful to Christoph Holder, Annekathrin Lincke, Doris Forderung and Elke Birkelbach for technical assistance, and to Rose Schmitz for helpful suggestions in performing the statistical analysis. This work was supported, in part, by the DFG (Ei 296/10–3).
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