Register      Login
Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Shopping Cart: ( empty )
You are here: Home > Journals > RD > RD07178
RESEARCH ARTICLE
Contents Vol 20(2)

Acrosome stability in the spermatozoa of dasyurid marsupials

N. A. Czarny A B , K. E. Mate A and J. C. Rodger A
+ Author Affiliations
- Author Affiliations

A Marsupial Research Laboratory, The University of Newcastle, Callaghan, NSW 2308, Australia.

B Corresponding author. Email: natasha.czarny@studentmail.newcastle.edu.au

Reproduction, Fertility and Development 20(2) 295-302 https://doi.org/10.1071/RD07178
Submitted: 28 September 2007  Accepted: 11 November 2007   Published: 24 January 2008

Abstract

The spermatozoa of most marsupials lack nuclear stabilising disulfide-bonded protamines found in eutherian mammals. However, disulfide stabilisation has been observed in the acrosome of macropodid (Macropus eugenii) and phalangerid (Trichosurus vulpecula) marsupials. As a result this organelle, which is normally fragile in eutherian mammals, is robust and able to withstand physical and chemical challenge in these marsupials. The present study examined acrosomal characteristics of the spermatozoa of three dasyurid marsupials; the fat-tailed dunnart (Sminthopsis crassicaudata), eastern quoll (Dasyurus viverrinus) and northern quoll (Dasyurus hallucatus). In all species examined Bryan’s staining demonstrated that significant acrosomal loss occurred following physical challenge with osmotic stress, cryopreservation without cryoprotectant and exposure to detergent (Triton-X). Bromobimane staining indicated that the acrosomes of dasyurids lacked stabilising disulfide bonds. As reported for the wallaby and possum, calcium ionophore (A23187) did not induce the acrosome reaction-like exocytosis in dasyurid spermatozoa but treatment with diacylglycerol (DiC8) caused significant acrosome loss at concentrations similar to those effective for other marsupials. The present study found that the spermatozoa of dasyurids are more sensitive to physical challenge than the previously-studied marsupials and we suggest that this is due to the absence of acrosomal stabilising disulfide bonds.


Acknowledgements

We thank Australian Ecosystems Foundation, Remabi Park and Territory Wildlife Park for the supply of eastern and northern quolls. This research was supported by the University of Newcastle. Woolworths Ltd Jesmond store generously donated meat to feed the eastern quolls. NC was the recipient of a University of Newcastle Research Scholarship.


References

Australian Federal Government (1999). Environment Protection and Biodiversity Conservation Act. Department of the Environment and Heritage, Canberra.

Balhorn, R. (1982). A model for the structure of chromatin in mammalian sperm. J. Cell Biol. 93, 298–305.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Czarny N. A., Harris M. S., and Rodger J. C. (2007). Preservation of spermatozoa from dasyurid marsupials. In ‘Proceedings of the Society of Reproductive Biologists Annual Scientific Meeting’. (Society for Reproductive Biology: Christchurch.)

Didion, B. A. , Dobrinsky, J. R. , Giles, J. R. , and Graves, C. N. (1989). Staining procedure to detect viability and the true acrosome reaction in spermatozoa of various species. Gamete Res. 22, 51–57.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Eddy E. M., and O’Brien D. A. (1994). The spermatozoon. In ‘The Physiology of Reproduction’. (Eds E. Knobil and J. D. Neill.) pp. 29–77. (Raven Press: New York.)

Fifis, T. , Cooper, D. W. , and Hill, R. J. (1990). Characterization of the protamines of the tammar wallaby (Macropus eugenii). Comp. Biochem. Physiol. B Biochem. Mol. Biol. 95, 571–575.
| Crossref | GoogleScholarGoogle Scholar | Mate K. E., Harris M. S., and Rodger J. C. (2000). Fertilization in monotreme, marsupial and eutherian mammals. In ‘Fertilization in Protozoa and Metazoan Animals’. (Eds J. J. Tarin and A. Cano.) pp. 223–275. (Springer-Verlag: Berlin.)

McClean, R. V. , Holt, W. V. , and Johnston, S. D. (2007). Ultrastructural observations of cryoinjury in kangaroo spermatozoa. Cryobiology 54, 271–280.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Penfold L. M., and Watson P. F. (2001). The cryopreservation of gametes and embryos of cattle, sheep, goats and pigs. In ‘Cryobanking the Genetic Resource’. (Eds P. F. Watson and W. V. Holt.) pp. 281–315. (Taylor & Francis: Great Britain.)

Richings, N. M. , Shaw, G. , Temple-Smith, P. D. , and Renfree, M. B. (2004). Intra-cytoplasmic sperm injection in a marsupial. Reproduction 128, 595–605.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Yanagimachi R. (1994) Mammalian fertilization. In ‘The Physiology of Reproduction’. (Eds E. Knobil and J. D. Neill.) pp. 189–318. (Raven Press: New York.)