Identification of genes regulated by an interaction between αvβ3 integrin and vitronectin in murine decidua
S. S. Mangale A , D. N. Modi B and K. V. R. Reddy A CA Department of Immunology, National Institute for Research in Reproductive Health (ICMR), J. M. Street, Parel, Mumbai 400012, India.
B Department of Stem Cell Biology, National Institute for Research in Reproductive Health (ICMR), J. M. Street, Parel, Mumbai 400012, India.
C Corresponding author. Email: shrichi@rediffmail.com
Reproduction, Fertility and Development 20(2) 311-319 https://doi.org/10.1071/RD07155
Submitted: 10 September 2007 Accepted: 15 November 2007 Published: 24 January 2008
Abstract
The delicate balance between embryo invasion and suppression of maternal immune rejection requires a fully functional decidua in species with haemochorial placenta. Our understanding of the decidual function is very limited due to the molecular and cellular complexity involved in decidualisation. The cell adhesion molecule αvβ3 integrin and its ligand vitronectin are upregulated in the mouse decidua during mid-pregnancy. The implications of interactions between αvβ3 and vitronectin in regulating decidual function are not known. In the present study, interactions between αvβ3 and vitronectin in the decidual cells of the mouse were blocked in vitro and effects on cell fate were evaluated by studying the differentially regulated genes by cDNA array and real-time polymerase chain reaction (PCR). The results indicate that expression of various genes involved in apoptotic and cell cycle pathways, as well as cytokine receptors, was deranged. Signalling through αvβ3 seems to be important to maintain a balance between cell proliferation and apoptosis, along with the modulation of inflammatory responses of decidual cells.
Additional keywords: cDNA array, mouse, pregnancy.
Acknowledgements
The present study was funded by the Indian Council of Medical Research. The authors are grateful to Dr Chander P. Puri (Director, NIRRH) for support and encouragement. SSM was supported by the Council for Scientific and Industrial Research (India) with a Senior Research Fellowship.
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