Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Shopping Cart: ( empty )
You are here: Home > Journals > RD > RD06143
RESEARCH ARTICLE
Contents Vol 19(3)

Expression pattern of integrins and their ligands in mouse feto-maternal tissues during pregnancy

Sachin S. Mangale A and K. V. R. Reddy A B
+ Author Affiliations
- Author Affiliations

A Immunology Division, National Institute for Research in Reproductive Health, J. M. Street, Parel, Mumbai 400 012, India.

B Corresponding author. Email: shrichi@rediffmail.com

Reproduction, Fertility and Development 19(3) 452-460 https://doi.org/10.1071/RD06143
Submitted: 24 October 2006  Accepted: 21 January 2007   Published: 14 March 2007

Abstract

The role of integrins, the cell-surface glycoproteins involved in various cellular functions, is well documented. However, information about their role and expression profile during pregnancy is still scant. In the present study, the expression of the integrin subunits β3, α6 and α5, along with their ligands vitronectin, osteopontin, laminin and fibronectin, was investigated in mouse uterus during different stages of pregnancy, namely 6.5, 8.5 and 13.5 days post coitus (d.p.c.) by immunohistochemical localisation. Integrins β3, α6 and α5 and the extracellular matrix molecules vitronectin and osteopontin exhibited dynamic spatiotemporal changes in their expression pattern in gestational endometrium, whereas fibronectin and laminin demonstrated more-or-less ubiquitous expression. The inter-implantation sites showed localisation of most of these molecules predominantly in the luminal epithelium, whereas their expression was negligible in the stroma. The present study explores the possible role and relevance of the spatiotemporal expression of integrins and their ligands in endometrial/decidual function and the maintenance of pregnancy.

Additional keywords: decidua, embryo, extracellular matrix, placenta.


Acknowledgements

The present study (NIRRH/MS/12/2006) was funded by the Indian Council of Medical Research. The authors express their gratitude to Dr Chander P. Puri (Director, NIRRH) for support and encouragement. Sachin S. Mangale is grateful to the Council for Scientific and Industrial Research (India) for a Senior Research Fellowship.


References

Abrahamsohn, P. A. , and Zorn, T. M. T. (1993). Implantation and decidualization in rodents. J. Exp. Zool. 266, 603–628.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Guidice L. C. (1999). Potential biochemical markers of uterine receptivity. Hum. Rep. 14(Suppl. 2), 3–16.

Hapke, S. , Kessler, H. , Prada, N. A. , Benge, A. , Shmitt, M. , Lengyel, E. , and Reuning, U. (2001). Integrin αvβ3/vitronectin interaction affects expression of the urokinase system in human ovarian cancer cells. J. Biol. Chem. 276, 26 340–26 348.
| Crossref | GoogleScholarGoogle Scholar | PubMed | O'Regan, A. W., Chupp, G. L., Lowry, J. A., Goetschkes, M., Mulligan, N., and Berman, J. S. (1999). Osteopontin is associated with T cells in sarcoid granulomas and has T cell adhesive and cytokine-like properties in vitro. J. Immunol. 162, 1024–1031.

Reddy, K. V. R. , and Mangale, S. M. (2003). Integrin receptors: the dynamic modulators of endometrial function. Tissue Cell 35, 260–273.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Reddy, K. V. R. , and Meherji, P. K. (1999). Integrin cell-adhesion molecules in endometrium of fertile and infertile women throughout the menstrual cycle. Indian J. Exp. Biol. 37, 323–331.
| PubMed |

Rouslahti, E. (1991). Integrins. J. Clin. Invest. 87, 1–5.
| PubMed |

Sekimoto, H. , Eipper-Mains, J. , Pond-Tor, S. , and Boney, C. M. (2005). αvβ3 integrins and Pyk2 mediate insulin-like growth factor I activation of Src and mitogen-activated protein kinase in 3T3-L1 cells. Mol. Endocrinol. 19, 1859–1867.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Shih, I. E. M. , and Kurman, R. J. (2002). Molecular basis of gestational trophoblastic diseases. Curr. Mol. Med. 2, 1–12.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Stupack, D. G. , Puente, X. S. , Boutsaboualoy, S. , Storgard, C. M. , and Cheresh, D. A. (2001). Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins. J. Cell Biol. 155, 459–470.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Tabibzadeh, S. (1992). Patterns of expression of integrin molecules in human endometrium throughout the menstrual cycle. Hum. Reprod. 7, 876–888.
| PubMed |

Zheng, B. , and Clemmons, D. R. (1998). Blocking ligand occupancy of the αvβ3 integrin inhibits insulin-like growth factor 1 signaling in vascular smooth muscle cells. Proc. Natl Acad. Sci. USA 95, 11 217–11 222.


Zheng, X. , Saunders, T. L. , Camper, S. A. , Samuelson, L. C. , and Ginsburg, D. (1995). Vitronectin is not essential for normal mammalian development and fertility. Proc. Natl Acad. Sci. USA 92, 12 426–12 430.
| Crossref | GoogleScholarGoogle Scholar | PubMed |