Genome multiplication is a generalised phenomenon in placentomal and interplacentomal trophoblast giant cells in cattle
Karl Klisch A A , Preben D. Thomsen B , Vibeke Dantzer B and Rudolf Leiser C
+ Author Affiliations
- Author Affiliations
A Microscopical Anatomy, Medical School of Hannover, 30623 Hannover, Germany.
B Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
C Institute of Veterinary Anatomy, Justus-Liebig-University Giessen, Germany.
D To whom correspondence should be addressed. email: klisch.karl@mh-hannover.de
Reproduction, Fertility and Development 16(3) 301-306 https://doi.org/10.1071/RD03101
Submitted: 31 October 2003 Accepted: 18 February 2004 Published: 8 April 2004
Abstract
The frequency of polyploidisation in bovine binucleate trophoblast giant cells (TGC) from placentomes (PL) and the interplacentomal allantochorion (AL) of six male fetuses with a crown–rump length between 3.5 and 103 cm was determined by in situ hybridisation with a chromosome-7-specific probe, using a probe specific for the Y chromosome to distinguish between maternal and fetal nuclei. The results showed that polyploid nuclei were essentially always of fetal origin. The frequency of tetraploid nuclei varied between 3% and 15% in both the placentomal and interplacentomal samples, with mean frequencies of 8.8% and 10.0% respectively. Octoploid nuclei were observed with a mean frequency of 1.1% in the interplacentomal samples, but were absent in samples from placentomes. Subsequent determination of nuclear DNA content by cytophotometric measurement of Feulgen-stained nuclei revealed that the frequency of nuclei with an 8C DNA content was several fold higher (AL 5.4%; PL 7.8%) than the frequency of octoploidy, suggesting that tetraploid TGC cells are arrested in the G2 phase of the cell cycle.
Extra keywords: cow, placenta, placentation, polyploidy.
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