Serial analysis of gene expression (SAGE) during porcine embryo development
Le Ann Blomberg A B and Kurt A. Zuelke A
+ Author Affiliations
- Author Affiliations
A Biotechnology and Germplasm Laboratory, Animal and Natural Resources Institute, USDA Agricultural Research Service, Beltsville, MD 20705, USA.
B To whom correspondence should be addressed. email: lblomberg@anri.barc.usda.gov
Reproduction, Fertility and Development 16(2) 87-92 https://doi.org/10.1071/RD03081
Submitted: 1 August 2003 Accepted: 1 October 2003 Published: 1 October 2003
Abstract
Functional genomics provides a powerful means for delving into the molecular mechanisms involved in pre-implantation development of porcine embryos. High rates of embryonic mortality (30%), following either natural mating or artificial insemination, emphasise the need to improve the efficiency of reproduction in the pig. The poor success rate of live offspring from in vitro-manipulated pig embryos also hampers efforts to generate transgenic animals for biotechnology applications. Previous analysis of differential gene expression has demonstrated stage-specific gene expression for in vivo-derived embryos and altered gene expression for in vitro-derived embryos. However, the methods used to date examine relatively few genes simultaneously and, thus, provide an incomplete glimpse of the physiological role of these genes during embryogenesis. The present review will focus on two aspects of applying functional genomics research strategies for analysing the expression of genes during elongation of pig embryos between gestational day (D) 11 and D12. First, we compare and contrast current methodologies that are being used for gene discovery and expression analysis during pig embryo development. Second, we establish a paradigm for applying serial analysis of gene expression as a functional genomics tool to obtain preliminary information essential for discovering the physiological mechanisms by which distinct embryonic phenotypes are derived.
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