Physiological limits to further improvement in the efficiency of oestrous synchronization in goats

Abstract

The variability between animals in the timing of oestrus after administration of a synchronization treatment seems to explain the low rate of fertility in goats inseminated at a predetermined time after progesterone withdrawal. Two experiments were performed during the breeding season to test whether the variation was due to the exogenous hormone regime or to the endogenous physiology of the animals. Twenty-one goats were given a synchronization treatment consisting of a vaginal sponge impregnated with 45 mg of fluorogestone acetate (FGA) for 11 days associated with intramuscular injection of 400 I.U. of equine chorionic gonadotrophin (eCG) and 50 µg of cloprostenol 48 h before sponge removal. Progesterone concentrations were measured during the subsequent cycle and the patterns were modelled to allow precise determination of the onset of luteolysis. Oestrus and the luteinizing hormone (LH) surge began 33·0 ± 6·8 h and 76·0 ± 33·0 h after sponge withdrawal, v. 43·4 ±5·7 h and 90·0 ± 36·0 h after natural luteolysis. For both observations, the between-goat variability was larger during the natural than during the synchronized oestrus (P < 0·05). The duration of the oestrous cycle was independent of the number of corpora lutea (CL), whereas the duration of luteal phase was shorter in goats with 2–3 CL (16·4 ± 0·9 day than in those with 1 CL: 17·7 ± 1·3 day; P < 0·05).

In the second experiment, 20 goats were ovariectomized and given a vaginal sponge as described above. Sixteen h after sponge removal, they were injected with 50 µg of oestradiol benzoate (ODB). This treatment was repeated with the second sponge being inserted 1–2 days after observation of oestrus. Oestrus and LH surge were observed: 32·8 ± 6·8 h v.27·8 ± 7·8 h after the first ODB injection, and 36·6 ± 7·3 h v. 34·3 ± 4·8 h after the second ODB injection. No relationship was observed between data of the two experiments. In both cases, the variability in the occurrence of oestrus and LH surge was of the same order as observed in the first experiment. This study shows that the timing of oestrus and LH surge is less variable after progestagen treatment than during a natural oestrous cycle. Moreover, a significant proportion of variability is inherent in the delays following the oestradiol peak, suggesting that further improvement in the synchronizing capacity of treatment based on progestagen administration is unlikely. Extra keyword: progestagens.