Measuring of ATP bioluminescence as a means of assessing washer disinfector performance and potentially as a means of validating the decontamination process
Raymond Heathcote A C and Brett Stadelmann BA Epworth Freemasons Hospital, Clarendon Street, East Melbourne, Vic. 3002, Australia.
B Central Sterile Supply Department, Epworth Freemasons Hospital, Clarendon Street, East Melbourne, Vic. 3002, Australia.
C Corresponding author. Email: raymond.heathcote@epworth.org.au
Healthcare Infection 14(4) 147-151 https://doi.org/10.1071/HI09019
Published: 21 December 2009
Abstract
This paper reports on the results of using a commercially available adenosine tri-phosphate bioluminescence (ATP) hand-held device for assessing the level of contamination on surgical instruments throughout the decontamination process. The report demonstrates that this technique may be a reliable point-of-use method for quantitatively assessing washer disinfector function and possibly a means of performing a washer disinfector validation process.
[1]
[2]
[3] Obee P, Griffith C, Cooper R, Cooke R, Bennion N, Lewis M. Real time monitoring in managing the decontamination of flexible gastrointestinal endoscopes. Am J Infect Control 2005; 33 202–6.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
[4] Hansen D, Hilgenhöner M, Popp W. ATP bioluminescence – for kitchen hygiene and cleaning control of surgical instruments. Intern J Infect Control 2008; 4 1–4.
| Crossref | GoogleScholarGoogle Scholar |
[5] Murdoch H, Taylor D, Dickinson J, Walker JT, Perrett D, Raven NDH, et al. Surface decontamination of surgical instruments: an ongoing dilemma. J Hosp Infect 2006; 63 432–8.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |
[6]
[7] Jimenez L. Adenosine triphosphate bioluminescence analysis for rapid screening of microbial contamination in non-sterile pharmaceutical samples. PDA J Pharm Sci Technol 2004; 58(3): 159–68.
| CAS | PubMed |
[8] Aycicek H, Oguz U, Karci K. Comparison of results of ATP bioluminescence and traditional hygiene swabbing methods for the determination of surface cleanliness at a hospital kitchen. Int J Hyg Environ Health 2006; 209(2): 203–6.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |
[9] Lewis T, Griffith C, Gallo M, Weinbren M. A modified ATP benchmark for evaluating the cleaning of some hospital environmental surfaces. J Hosp Infect 2008; 69 156–63.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |
[10] Andersen BM, Rasch M, Kvist J, Tollefsen T, Lukkassen R, Sandvik L, et al. Floor cleaning: effect on bacteria and organic materials in hospital rooms. J Hosp Infect 2009; 71(1): 57–65.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |
[11]