In silico characterisation and functional validation of chilling tolerant divergence 1 (COLD1) gene in monocots during abiotic stress
P. Anunathini A , V. M. Manoj B , T. S. Sarath Padmanabhan B , S. Dhivya A , J. Ashwin Narayan B , C. Appunu B C and R. Sathishkumar A C
+ Author Affiliations
- Author Affiliations
A Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, India.
B Genetic Transformation Lab, Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, India.
C Corresponding authors. Emails: rsathish@buc.edu.in; cappunu@gmail.com
Functional Plant Biology 46(6) 524-532 https://doi.org/10.1071/FP18189
Submitted: 16 July 2018 Accepted: 24 January 2019 Published: 4 March 2019
Abstract
The G protein-coupled receptor is one of the major transmembrane proteins in plants. It consists of an α subunit, a β subunit and three γ subunits. Chilling tolerant divergence 1 (COLD1) includes a Golgi pH receptor (GPHR) domain, which maintains cell membrane organisation and dynamics, along with abscisic acid linked G protein-coupled receptor (ABA_GPCR) that regulates the signalling pathways during cold stress. In the present study, we performed characterisation of a homologous COLD1 from the economically important monocot species Oryza sativa L., Zea mays L., Sorghum bicolor (L.) Moench and Erianthus arundinaceus (L.) Beauv. IK 76-81, a wild relative of Saccharum. COLD1 was isolated from E. arundinaceus IK 76-81, analysed for its evolution, domain, membrane topology, followed by prediction of secondary, tertiary structures and functionally validated in all four different monocots. Gene expression studies of COLD1 revealed differential expression under heat, drought, salinity and cold stresses in selected monocots. This is the first study on regulation of native COLD1 during abiotic stress in monocots, which has opened up new leads for trait improvement strategies in this economically important crop species.
Additional keywords: abiotic stress, bioinformatics, proline, qRT-PCR.
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