Cycloheximide Inhibition of Cytokinin-dependent Protein Synthesis: Correlation with Betacyanin Synthesis

Abstract

Cytokinin-dependent betacyanin accumulation in Amaranthus trlcolor half-seedlings (cotyledons plus 5-mm hypocotyls) can be detected after a lag period of about 6 h. A significant (P<O.01) promotion by cytokinin of [3H]leucine incorporation into protein was observed at 5-7 h. It is concluded that the cytokinin-induced biological response was subsequent to or at the same time as the effect on protein synthesis and therefore could be dependent on it, a conclusion that is strengthened by the correlation between the effects of cycloheximide on the two processes. Cycloheximide preferentially inhibits cytokinin-dependent protein synthesis as compared to the effect of the inhibitor on the basal rate.