Induction and Repression of Acetamidase in Chlamydomonas veinhavdi

Abstract

Using a static induction approach it was shown that acetamidase of Chlamydomonas reinhardi was regulated by ammonium repression and acetamidase induction. Urea, thiourea and acetamide were efficient inducing substances; formamide, fluoroacetamide and acrylamide, although acting as substrates, failed to induce the enzyme. Derepressed levels of acetamidase were shown to yield sufficient nitrogen for growth, with amides giving high maximum enzyme velocities. Inhibitor studies showed that derepressed and induced enzyme levels require transcription and translation.